A Novel Capillary Electrophoresis-Based High-Throughput Multiplex Polymerase Chain Reaction System for the Simultaneous Detection of Nine Pathogens in Swine
Joint Authors
Wu, Xu-long
Xiao, Lu
Lin, Hua
Yang, Miao
Chen, Shi-jie
An, Wei
Wang, Yin
Yao, Xue-ping
Yang, Ze-xiao
Tang, Zi-zhong
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Here we aimed to develop a capillary electrophoresis-based high-throughput multiplex polymerase chain reaction (PCR) system for the simultaneous detection of nine pathogens in swine.
Nine pairs of specific primers and a set of universal primers were designed; the multiplex PCR was established.
The specificity and cross-reactivity of this assay were examined, and the detection limit was determined using serial 10-fold dilutions of plasmids containing the target sequences.
The assay was further tested using 144 clinical samples.
We found that the nine specific amplification peaks were observed, and the assay had a high degree of specificity, without nonspecific amplification.
The simultaneous detection limit for the nine viruses reached 10000 copies μL−1 when all of the premixed viral targets were present.
Seventy-seven of the clinical samples tested positive for at least one of the viruses; the principal viral infections in the clinical samples were porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus.
This approach has much potential for further development of high-throughput detection tools for the diagnosis of diseases in animals.
American Psychological Association (APA)
Wu, Xu-long& Xiao, Lu& Lin, Hua& Yang, Miao& Chen, Shi-jie& An, Wei…[et al.]. 2017. A Novel Capillary Electrophoresis-Based High-Throughput Multiplex Polymerase Chain Reaction System for the Simultaneous Detection of Nine Pathogens in Swine. BioMed Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1138471
Modern Language Association (MLA)
Wu, Xu-long…[et al.]. A Novel Capillary Electrophoresis-Based High-Throughput Multiplex Polymerase Chain Reaction System for the Simultaneous Detection of Nine Pathogens in Swine. BioMed Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1138471
American Medical Association (AMA)
Wu, Xu-long& Xiao, Lu& Lin, Hua& Yang, Miao& Chen, Shi-jie& An, Wei…[et al.]. A Novel Capillary Electrophoresis-Based High-Throughput Multiplex Polymerase Chain Reaction System for the Simultaneous Detection of Nine Pathogens in Swine. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1138471
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138471