Differential S1P Receptor Profiles on M1- and M2-Polarized Macrophages Affect Macrophage Cytokine Production and Migration
المؤلفون المشاركون
Heidecke, Claus-Dieter
Schulze, Tobias
Müller, Jan
von Bernstorff, Wolfram
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-03-06
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Introduction.
Macrophages are key players in complex biological processes.
In response to environmental signals, macrophages undergo polarization towards a proinflammatory (M1) or anti-inflammatory (M2) phenotype.
Sphingosine 1-phosphate (S1P) is a bioactive lysophospholipid that acts via 5 G-protein coupled receptors (S1P1–5) in order to influence a broad spectrum of biological processes.
This study assesses S1P receptor expression on macrophages before and after M1 and M2 polarization and performs a comparative analysis of S1P signalling in the two activational states of macrophages.
Methods.
Bone marrow derived macrophages (BMDM) from C57 BL/6 mice were cultured under either M1- or M2-polarizing conditions.
S1P-receptor expression was determined by quantitative RT-PCR.
Influence of S1P on macrophage activation, migration, phagocytosis, and cytokine secretion was assessed in vitro.
Results.
All 5 S1P receptor subclasses were expressed in macrophages.
Culture under both M1- and M2-polarizing conditions led to significant downregulation of S1P1.
In contrast, M1-polarized macrophages significantly downregulated S1P4.
The expression of the remaining three S1P receptors did not change.
S1P increased expression of iNOS under M2-polarizing conditions.
Furthermore, S1P induced chemotaxis in M1 macrophages and changed cytokine production in M2 macrophages.
Phagocytosis was not affected by S1P-signalling.
Discussion.
The expression of different specific S1P receptor profiles may provide a possibility to selectively influence M1- or M2-polarized macrophages.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Müller, Jan& von Bernstorff, Wolfram& Heidecke, Claus-Dieter& Schulze, Tobias. 2017. Differential S1P Receptor Profiles on M1- and M2-Polarized Macrophages Affect Macrophage Cytokine Production and Migration. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1138676
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Müller, Jan…[et al.]. Differential S1P Receptor Profiles on M1- and M2-Polarized Macrophages Affect Macrophage Cytokine Production and Migration. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1138676
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Müller, Jan& von Bernstorff, Wolfram& Heidecke, Claus-Dieter& Schulze, Tobias. Differential S1P Receptor Profiles on M1- and M2-Polarized Macrophages Affect Macrophage Cytokine Production and Migration. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1138676
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1138676
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر