Differential S1P Receptor Profiles on M1- and M2-Polarized Macrophages Affect Macrophage Cytokine Production and Migration
Joint Authors
Heidecke, Claus-Dieter
Schulze, Tobias
Müller, Jan
von Bernstorff, Wolfram
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Introduction.
Macrophages are key players in complex biological processes.
In response to environmental signals, macrophages undergo polarization towards a proinflammatory (M1) or anti-inflammatory (M2) phenotype.
Sphingosine 1-phosphate (S1P) is a bioactive lysophospholipid that acts via 5 G-protein coupled receptors (S1P1–5) in order to influence a broad spectrum of biological processes.
This study assesses S1P receptor expression on macrophages before and after M1 and M2 polarization and performs a comparative analysis of S1P signalling in the two activational states of macrophages.
Methods.
Bone marrow derived macrophages (BMDM) from C57 BL/6 mice were cultured under either M1- or M2-polarizing conditions.
S1P-receptor expression was determined by quantitative RT-PCR.
Influence of S1P on macrophage activation, migration, phagocytosis, and cytokine secretion was assessed in vitro.
Results.
All 5 S1P receptor subclasses were expressed in macrophages.
Culture under both M1- and M2-polarizing conditions led to significant downregulation of S1P1.
In contrast, M1-polarized macrophages significantly downregulated S1P4.
The expression of the remaining three S1P receptors did not change.
S1P increased expression of iNOS under M2-polarizing conditions.
Furthermore, S1P induced chemotaxis in M1 macrophages and changed cytokine production in M2 macrophages.
Phagocytosis was not affected by S1P-signalling.
Discussion.
The expression of different specific S1P receptor profiles may provide a possibility to selectively influence M1- or M2-polarized macrophages.
American Psychological Association (APA)
Müller, Jan& von Bernstorff, Wolfram& Heidecke, Claus-Dieter& Schulze, Tobias. 2017. Differential S1P Receptor Profiles on M1- and M2-Polarized Macrophages Affect Macrophage Cytokine Production and Migration. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1138676
Modern Language Association (MLA)
Müller, Jan…[et al.]. Differential S1P Receptor Profiles on M1- and M2-Polarized Macrophages Affect Macrophage Cytokine Production and Migration. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1138676
American Medical Association (AMA)
Müller, Jan& von Bernstorff, Wolfram& Heidecke, Claus-Dieter& Schulze, Tobias. Differential S1P Receptor Profiles on M1- and M2-Polarized Macrophages Affect Macrophage Cytokine Production and Migration. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1138676
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1138676