Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats
المؤلفون المشاركون
Joo, Hyun-Woo
Park, In-Hwa
Shen, Guang-Yin
Seong, Jin-Hee
Shin, Na-Kyoung
Lee, A-Hyeon
Kim, Kyung-Soo
Song, Yi-Sun
Kim, Hyuck
المصدر
Canadian Journal of Gastroenterology and Hepatology
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-07-25
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Background.
We previously reported that the granulocyte colony stimulating factor (G-CSF) ameliorated hepatic steatosis with the enhancement of β-oxidation-related gene expression.
However, the mechanisms underlying this process remain unclear.
This study aimed to determine whether the improvement of hepatic steatosis by G-CSF was associated with autophagy in a rat model of diabetes.
Methods.
Eight rats were fed a standard diet, and 16 rats were fed high-fat diet (HFD) for 5 weeks.
All HFD-fed rats were then injected with streptozotocin (STZ).
One week later, HFD rats injected with STZ were randomly treated with either G-CSF (200 μg/kg/day; diabetes mellitus (DM)/G-CSF) or saline (DM/saline) for 5 consecutive days.
Four weeks later, serum biochemical and histology analyses were conducted.
The expression of autophagy-associated proteins was determined by Western blotting.
The mRNA expression of β-oxidation-related genes was determined by quantitative real-time polymerase chain reaction.
HepG2 cells were cultured under high glucose (HG) conditions with G-CSF treatment, followed by Oil Red O staining for quantification of lipids.
Results.
Histological analysis showed lower lipid accumulation in the DM/G-CSF group than in the DM/saline-treated rats.
Protein levels of LC3 and beclin-1 were higher, and those of p62 were lower in the DM/G-CSF rats than in the DM/saline-treated rats.
The mRNA expression of β-oxidation-related genes was higher in DM/G-CSF rats than in the DM/saline-treated rats.
Quantification of lipid levels in HepG2 cells cultured with HG and G-CSF treatment revealed no significant differences.
Conclusions.
Our data suggested that G-CSF potentially improves hepatic steatosis and autophagy in the liver of diabetic rats.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Joo, Hyun-Woo& Song, Yi-Sun& Park, In-Hwa& Shen, Guang-Yin& Seong, Jin-Hee& Shin, Na-Kyoung…[et al.]. 2020. Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats. Canadian Journal of Gastroenterology and Hepatology،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1139001
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Joo, Hyun-Woo…[et al.]. Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats. Canadian Journal of Gastroenterology and Hepatology No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1139001
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Joo, Hyun-Woo& Song, Yi-Sun& Park, In-Hwa& Shen, Guang-Yin& Seong, Jin-Hee& Shin, Na-Kyoung…[et al.]. Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats. Canadian Journal of Gastroenterology and Hepatology. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1139001
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1139001
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر