Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats
Joint Authors
Joo, Hyun-Woo
Park, In-Hwa
Shen, Guang-Yin
Seong, Jin-Hee
Shin, Na-Kyoung
Lee, A-Hyeon
Kim, Kyung-Soo
Song, Yi-Sun
Kim, Hyuck
Source
Canadian Journal of Gastroenterology and Hepatology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-25
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
We previously reported that the granulocyte colony stimulating factor (G-CSF) ameliorated hepatic steatosis with the enhancement of β-oxidation-related gene expression.
However, the mechanisms underlying this process remain unclear.
This study aimed to determine whether the improvement of hepatic steatosis by G-CSF was associated with autophagy in a rat model of diabetes.
Methods.
Eight rats were fed a standard diet, and 16 rats were fed high-fat diet (HFD) for 5 weeks.
All HFD-fed rats were then injected with streptozotocin (STZ).
One week later, HFD rats injected with STZ were randomly treated with either G-CSF (200 μg/kg/day; diabetes mellitus (DM)/G-CSF) or saline (DM/saline) for 5 consecutive days.
Four weeks later, serum biochemical and histology analyses were conducted.
The expression of autophagy-associated proteins was determined by Western blotting.
The mRNA expression of β-oxidation-related genes was determined by quantitative real-time polymerase chain reaction.
HepG2 cells were cultured under high glucose (HG) conditions with G-CSF treatment, followed by Oil Red O staining for quantification of lipids.
Results.
Histological analysis showed lower lipid accumulation in the DM/G-CSF group than in the DM/saline-treated rats.
Protein levels of LC3 and beclin-1 were higher, and those of p62 were lower in the DM/G-CSF rats than in the DM/saline-treated rats.
The mRNA expression of β-oxidation-related genes was higher in DM/G-CSF rats than in the DM/saline-treated rats.
Quantification of lipid levels in HepG2 cells cultured with HG and G-CSF treatment revealed no significant differences.
Conclusions.
Our data suggested that G-CSF potentially improves hepatic steatosis and autophagy in the liver of diabetic rats.
American Psychological Association (APA)
Joo, Hyun-Woo& Song, Yi-Sun& Park, In-Hwa& Shen, Guang-Yin& Seong, Jin-Hee& Shin, Na-Kyoung…[et al.]. 2020. Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats. Canadian Journal of Gastroenterology and Hepatology،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1139001
Modern Language Association (MLA)
Joo, Hyun-Woo…[et al.]. Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats. Canadian Journal of Gastroenterology and Hepatology No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1139001
American Medical Association (AMA)
Joo, Hyun-Woo& Song, Yi-Sun& Park, In-Hwa& Shen, Guang-Yin& Seong, Jin-Hee& Shin, Na-Kyoung…[et al.]. Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats. Canadian Journal of Gastroenterology and Hepatology. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1139001
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139001