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Determination of Aflatoxin M1 and Chloramphenicol in Milk Based on Background Fluorescence Quenching Immunochromatographic Assay
المؤلفون المشاركون
Wu, Xiaoxia
Tian, Xiaofeng
Xu, Lihua
Li, Jiutong
Li, Xinxia
Wang, Yuwen
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-03-06
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Harsh demanding has been exposed on the concentration of aflatoxin M1 (AFM1) and chloramphenicol (CAP) in milk.
In this study, we developed a new method based on background fluorescence quenching immunochromatographic assay (bFQICA) to detect AFM1 and CAP in milk.
The detection limit for AFM1 was 0.0009 ng/mL, while that for the CAP was 0.0008 ng/mL.
The assay variability was determined with 3 AFM1 standards (i.e., 0.25 ng/mL, 0.5 ng/mL, and 1.0 ng/mL), and the actual detection value was 0.2497, 0.5329, and 1.0941, respectively.
For the assay variability of 3 CAP standards (i.e., 0.10 ng/mL, 0.30 ng/mL, and 0.50 ng/mL), the actual detection value was 0.0996, 0.3096, and 0.4905, respectively.
The recovery rate of AFM1 was 99.7%–101.7%, while that for CAP was 95.3%–97.6%.
For the test stability, AFM1 and CAP showed satisfactory test stability even at month 5.
Compared with the sensitivity of liquid chromatography-mass spectrometry (LC-MS) method, no statistical difference was noticed in results of the bFQICA.
Our method is convenient for the detection of AFM1 and CAP in milk with a test duration of about 8 minutes.
Additionally, an internal WiFi facility is provided in the system allowing for quick connection and storage in the intelligent cell phone.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wu, Xiaoxia& Tian, Xiaofeng& Xu, Lihua& Li, Jiutong& Li, Xinxia& Wang, Yuwen. 2017. Determination of Aflatoxin M1 and Chloramphenicol in Milk Based on Background Fluorescence Quenching Immunochromatographic Assay. BioMed Research International،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1139216
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wu, Xiaoxia…[et al.]. Determination of Aflatoxin M1 and Chloramphenicol in Milk Based on Background Fluorescence Quenching Immunochromatographic Assay. BioMed Research International No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1139216
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wu, Xiaoxia& Tian, Xiaofeng& Xu, Lihua& Li, Jiutong& Li, Xinxia& Wang, Yuwen. Determination of Aflatoxin M1 and Chloramphenicol in Milk Based on Background Fluorescence Quenching Immunochromatographic Assay. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1139216
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1139216
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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