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Determination of Aflatoxin M1 and Chloramphenicol in Milk Based on Background Fluorescence Quenching Immunochromatographic Assay
Joint Authors
Wu, Xiaoxia
Tian, Xiaofeng
Xu, Lihua
Li, Jiutong
Li, Xinxia
Wang, Yuwen
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-06
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Harsh demanding has been exposed on the concentration of aflatoxin M1 (AFM1) and chloramphenicol (CAP) in milk.
In this study, we developed a new method based on background fluorescence quenching immunochromatographic assay (bFQICA) to detect AFM1 and CAP in milk.
The detection limit for AFM1 was 0.0009 ng/mL, while that for the CAP was 0.0008 ng/mL.
The assay variability was determined with 3 AFM1 standards (i.e., 0.25 ng/mL, 0.5 ng/mL, and 1.0 ng/mL), and the actual detection value was 0.2497, 0.5329, and 1.0941, respectively.
For the assay variability of 3 CAP standards (i.e., 0.10 ng/mL, 0.30 ng/mL, and 0.50 ng/mL), the actual detection value was 0.0996, 0.3096, and 0.4905, respectively.
The recovery rate of AFM1 was 99.7%–101.7%, while that for CAP was 95.3%–97.6%.
For the test stability, AFM1 and CAP showed satisfactory test stability even at month 5.
Compared with the sensitivity of liquid chromatography-mass spectrometry (LC-MS) method, no statistical difference was noticed in results of the bFQICA.
Our method is convenient for the detection of AFM1 and CAP in milk with a test duration of about 8 minutes.
Additionally, an internal WiFi facility is provided in the system allowing for quick connection and storage in the intelligent cell phone.
American Psychological Association (APA)
Wu, Xiaoxia& Tian, Xiaofeng& Xu, Lihua& Li, Jiutong& Li, Xinxia& Wang, Yuwen. 2017. Determination of Aflatoxin M1 and Chloramphenicol in Milk Based on Background Fluorescence Quenching Immunochromatographic Assay. BioMed Research International،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1139216
Modern Language Association (MLA)
Wu, Xiaoxia…[et al.]. Determination of Aflatoxin M1 and Chloramphenicol in Milk Based on Background Fluorescence Quenching Immunochromatographic Assay. BioMed Research International No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1139216
American Medical Association (AMA)
Wu, Xiaoxia& Tian, Xiaofeng& Xu, Lihua& Li, Jiutong& Li, Xinxia& Wang, Yuwen. Determination of Aflatoxin M1 and Chloramphenicol in Milk Based on Background Fluorescence Quenching Immunochromatographic Assay. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1139216
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139216