Improved scFv Anti-LOX-1 Binding Activity by Fusion with LOX-1-Binding Peptides
المؤلفون المشاركون
Hu, Wei
Xie, Qiuhong
Xiang, Hongyu
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-09-28
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The oxidized low-density lipoprotein receptor-1 (LOX-1) targeted single-chain variable fragment (scFvs) is a promising molecule for the targeted delivery of imaging and therapeutic molecules of atherosclerotic diseases; however, its applications are limited by the inherent low antigen affinity.
In this study, the three-dimensional (3D) model of the anti-LOX-1 scFv was constructed and its docking with the LOX-1 protein was developed.
To improve the LOX-1-binding activity, the anti-LOX-1 scFv was designed to fuse with one of three LOX-1-binding heptapeptides, LTPATAI, FQTPPQL, and LSIPPKA, at its N-terminus and C-terminus and in the linker region, which have different LOX-1-binding interfaces with the anti-LOX-1 scFv analyzed by an array of computational approaches.
These scFv/peptide fusions were constructed, successfully expressed in Brevibacillus choshinensis hosts, and purified by a two-step column purification process.
The antigen binding activity, structural characteristics, thermal stability, and stability in serum of these fusion proteins were examined.
Results showed that the scFv with N-terminal fusing peptides proteins demonstrated increased LOX-1-binding activity without decrease in stability.
These findings will help increase the application efficacy of LOX-1 targeting scFv in LOX-1-based therapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hu, Wei& Xie, Qiuhong& Xiang, Hongyu. 2017. Improved scFv Anti-LOX-1 Binding Activity by Fusion with LOX-1-Binding Peptides. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139289
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hu, Wei…[et al.]. Improved scFv Anti-LOX-1 Binding Activity by Fusion with LOX-1-Binding Peptides. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1139289
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hu, Wei& Xie, Qiuhong& Xiang, Hongyu. Improved scFv Anti-LOX-1 Binding Activity by Fusion with LOX-1-Binding Peptides. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139289
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1139289
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر