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Improved scFv Anti-LOX-1 Binding Activity by Fusion with LOX-1-Binding Peptides
Joint Authors
Hu, Wei
Xie, Qiuhong
Xiang, Hongyu
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The oxidized low-density lipoprotein receptor-1 (LOX-1) targeted single-chain variable fragment (scFvs) is a promising molecule for the targeted delivery of imaging and therapeutic molecules of atherosclerotic diseases; however, its applications are limited by the inherent low antigen affinity.
In this study, the three-dimensional (3D) model of the anti-LOX-1 scFv was constructed and its docking with the LOX-1 protein was developed.
To improve the LOX-1-binding activity, the anti-LOX-1 scFv was designed to fuse with one of three LOX-1-binding heptapeptides, LTPATAI, FQTPPQL, and LSIPPKA, at its N-terminus and C-terminus and in the linker region, which have different LOX-1-binding interfaces with the anti-LOX-1 scFv analyzed by an array of computational approaches.
These scFv/peptide fusions were constructed, successfully expressed in Brevibacillus choshinensis hosts, and purified by a two-step column purification process.
The antigen binding activity, structural characteristics, thermal stability, and stability in serum of these fusion proteins were examined.
Results showed that the scFv with N-terminal fusing peptides proteins demonstrated increased LOX-1-binding activity without decrease in stability.
These findings will help increase the application efficacy of LOX-1 targeting scFv in LOX-1-based therapy.
American Psychological Association (APA)
Hu, Wei& Xie, Qiuhong& Xiang, Hongyu. 2017. Improved scFv Anti-LOX-1 Binding Activity by Fusion with LOX-1-Binding Peptides. BioMed Research International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139289
Modern Language Association (MLA)
Hu, Wei…[et al.]. Improved scFv Anti-LOX-1 Binding Activity by Fusion with LOX-1-Binding Peptides. BioMed Research International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1139289
American Medical Association (AMA)
Hu, Wei& Xie, Qiuhong& Xiang, Hongyu. Improved scFv Anti-LOX-1 Binding Activity by Fusion with LOX-1-Binding Peptides. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1139289
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139289