miR-152-3p Affects the Progression of Colon Cancer via the KLF4IFITM3 Axis
المؤلفون المشاركون
Zhu, Xiaoyi
Shen, Zhan
Man, Da
Ruan, Hang
Huang, Sha
المصدر
Computational and Mathematical Methods in Medicine
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-09-01
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Objective.
The purpose of this study was to investigate the relationship between miR-152-3p and the KLF4/IFITM3 axis, thereby revealing the mechanism underlying colon cancer occurrence and development, consequently providing a promising target for colon cancer treatment.
Methods.
Bioinformatics methods were implemented to analyze the differential expression of miRNAs and mRNAs in colon cancer, confirm the target miRNA, and predict the downstream targeted mRNAs.
qRT-PCR and Western blot were performed to detect the expression of miR-152-3p, KLF4, and IFITM3.
CCK-8 and colony formation assays were conducted for the assessment of cell proliferation, and flow cytometry was carried out for the detection of cell apoptosis.
Finally, dual-luciferase reporter gene assay was employed to verify the targeting relationship between miR-152-3p and KLF4.
Results.
miR-152-3p was highly expressed in colon cancer cells, whereas KLF4 was poorly expressed.
Dual-luciferase assay verified that miR-152-3p targeted to bind to KLF4 and suppressed its expression.
Moreover, silencing miR-152-3p or overexpressing KLF4 was found to downregulate IFITM3, thereby inhibiting cell proliferation and potentiating cell apoptosis.
In rescue experiments, we found that miR-152-3p deficiency decreased the expression of IFITM3 and weakened cancer cell proliferation, and such effects were restored when miR-152-3p and KLF4 were silenced simultaneously.
Conclusion.
In sum, we discovered that miR-152-3p can affect the pathogenesis of colon cancer via the KLF4/IFITM3 axis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhu, Xiaoyi& Shen, Zhan& Man, Da& Ruan, Hang& Huang, Sha. 2020. miR-152-3p Affects the Progression of Colon Cancer via the KLF4IFITM3 Axis. Computational and Mathematical Methods in Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1139605
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhu, Xiaoyi…[et al.]. miR-152-3p Affects the Progression of Colon Cancer via the KLF4IFITM3 Axis. Computational and Mathematical Methods in Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1139605
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhu, Xiaoyi& Shen, Zhan& Man, Da& Ruan, Hang& Huang, Sha. miR-152-3p Affects the Progression of Colon Cancer via the KLF4IFITM3 Axis. Computational and Mathematical Methods in Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1139605
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1139605
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر