miR-152-3p Affects the Progression of Colon Cancer via the KLF4IFITM3 Axis
Joint Authors
Zhu, Xiaoyi
Shen, Zhan
Man, Da
Ruan, Hang
Huang, Sha
Source
Computational and Mathematical Methods in Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objective.
The purpose of this study was to investigate the relationship between miR-152-3p and the KLF4/IFITM3 axis, thereby revealing the mechanism underlying colon cancer occurrence and development, consequently providing a promising target for colon cancer treatment.
Methods.
Bioinformatics methods were implemented to analyze the differential expression of miRNAs and mRNAs in colon cancer, confirm the target miRNA, and predict the downstream targeted mRNAs.
qRT-PCR and Western blot were performed to detect the expression of miR-152-3p, KLF4, and IFITM3.
CCK-8 and colony formation assays were conducted for the assessment of cell proliferation, and flow cytometry was carried out for the detection of cell apoptosis.
Finally, dual-luciferase reporter gene assay was employed to verify the targeting relationship between miR-152-3p and KLF4.
Results.
miR-152-3p was highly expressed in colon cancer cells, whereas KLF4 was poorly expressed.
Dual-luciferase assay verified that miR-152-3p targeted to bind to KLF4 and suppressed its expression.
Moreover, silencing miR-152-3p or overexpressing KLF4 was found to downregulate IFITM3, thereby inhibiting cell proliferation and potentiating cell apoptosis.
In rescue experiments, we found that miR-152-3p deficiency decreased the expression of IFITM3 and weakened cancer cell proliferation, and such effects were restored when miR-152-3p and KLF4 were silenced simultaneously.
Conclusion.
In sum, we discovered that miR-152-3p can affect the pathogenesis of colon cancer via the KLF4/IFITM3 axis.
American Psychological Association (APA)
Zhu, Xiaoyi& Shen, Zhan& Man, Da& Ruan, Hang& Huang, Sha. 2020. miR-152-3p Affects the Progression of Colon Cancer via the KLF4IFITM3 Axis. Computational and Mathematical Methods in Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1139605
Modern Language Association (MLA)
Zhu, Xiaoyi…[et al.]. miR-152-3p Affects the Progression of Colon Cancer via the KLF4IFITM3 Axis. Computational and Mathematical Methods in Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1139605
American Medical Association (AMA)
Zhu, Xiaoyi& Shen, Zhan& Man, Da& Ruan, Hang& Huang, Sha. miR-152-3p Affects the Progression of Colon Cancer via the KLF4IFITM3 Axis. Computational and Mathematical Methods in Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1139605
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139605