The Effects of Ex Vivo Administration of Granulocyte-Macrophage Colony-Stimulating Factor and Endotoxin on Cytokine Release of Whole Blood Are Determined by Priming Conditions
المؤلفون المشاركون
Nierhaus, Axel
Linssen, J.
Winkler, M. S.
Frings, D. P.
Kluge, S.
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-12-14
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Background.
Lipopolysaccharide- (LPS-) induced tumour necrosis factor alpha (TNFα) secretion in critically ill patients can be considered as a measure of immune responsiveness.
It can be enhanced by granulocyte-macrophage colony stimulating factor (GM-CSF).
We investigated the effect of GM-CSF on ex vivo stimulated cytokine production using various preincubation regimens in healthy donors and patients with sepsis.
Results.
The maxima for the stimuli occurred 3 hours after stimulation.
In donors, there was an increase (p<0.001) of LPS-induced TNFα levels following incubation with GM-CSF.
The simultaneous incubation with GM-CSF and LPS caused an inhibition of TNFα production (p<0.001).
Postincubation with GM-CSF did not yield any difference.
In patients, preincubation with GM-CSF yielded an enhanced ex vivo TNFα-response when TNFα levels were low.
Patients with increased TNFα concentrations did not show a GM-CSF stimulation effect.
The GM-CSF preincubation yielded an increase of IL-8 production in patients and donors.
Conclusions.
This study demonstrates the immune-modulating properties of GM-CSF depending on the absence or presence of LPS or systemic TNFα.
The timing of GM-CSF administration may be relevant for the modulation of the immune system in sepsis.
The lack of stimulation in patients with high TNFα may represent endotoxin tolerance.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Nierhaus, Axel& Linssen, J.& Winkler, M. S.& Frings, D. P.& Kluge, S.. 2017. The Effects of Ex Vivo Administration of Granulocyte-Macrophage Colony-Stimulating Factor and Endotoxin on Cytokine Release of Whole Blood Are Determined by Priming Conditions. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139734
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Nierhaus, Axel…[et al.]. The Effects of Ex Vivo Administration of Granulocyte-Macrophage Colony-Stimulating Factor and Endotoxin on Cytokine Release of Whole Blood Are Determined by Priming Conditions. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1139734
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Nierhaus, Axel& Linssen, J.& Winkler, M. S.& Frings, D. P.& Kluge, S.. The Effects of Ex Vivo Administration of Granulocyte-Macrophage Colony-Stimulating Factor and Endotoxin on Cytokine Release of Whole Blood Are Determined by Priming Conditions. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139734
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1139734
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر