The Effects of Ex Vivo Administration of Granulocyte-Macrophage Colony-Stimulating Factor and Endotoxin on Cytokine Release of Whole Blood Are Determined by Priming Conditions
Joint Authors
Nierhaus, Axel
Linssen, J.
Winkler, M. S.
Frings, D. P.
Kluge, S.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Lipopolysaccharide- (LPS-) induced tumour necrosis factor alpha (TNFα) secretion in critically ill patients can be considered as a measure of immune responsiveness.
It can be enhanced by granulocyte-macrophage colony stimulating factor (GM-CSF).
We investigated the effect of GM-CSF on ex vivo stimulated cytokine production using various preincubation regimens in healthy donors and patients with sepsis.
Results.
The maxima for the stimuli occurred 3 hours after stimulation.
In donors, there was an increase (p<0.001) of LPS-induced TNFα levels following incubation with GM-CSF.
The simultaneous incubation with GM-CSF and LPS caused an inhibition of TNFα production (p<0.001).
Postincubation with GM-CSF did not yield any difference.
In patients, preincubation with GM-CSF yielded an enhanced ex vivo TNFα-response when TNFα levels were low.
Patients with increased TNFα concentrations did not show a GM-CSF stimulation effect.
The GM-CSF preincubation yielded an increase of IL-8 production in patients and donors.
Conclusions.
This study demonstrates the immune-modulating properties of GM-CSF depending on the absence or presence of LPS or systemic TNFα.
The timing of GM-CSF administration may be relevant for the modulation of the immune system in sepsis.
The lack of stimulation in patients with high TNFα may represent endotoxin tolerance.
American Psychological Association (APA)
Nierhaus, Axel& Linssen, J.& Winkler, M. S.& Frings, D. P.& Kluge, S.. 2017. The Effects of Ex Vivo Administration of Granulocyte-Macrophage Colony-Stimulating Factor and Endotoxin on Cytokine Release of Whole Blood Are Determined by Priming Conditions. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139734
Modern Language Association (MLA)
Nierhaus, Axel…[et al.]. The Effects of Ex Vivo Administration of Granulocyte-Macrophage Colony-Stimulating Factor and Endotoxin on Cytokine Release of Whole Blood Are Determined by Priming Conditions. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1139734
American Medical Association (AMA)
Nierhaus, Axel& Linssen, J.& Winkler, M. S.& Frings, D. P.& Kluge, S.. The Effects of Ex Vivo Administration of Granulocyte-Macrophage Colony-Stimulating Factor and Endotoxin on Cytokine Release of Whole Blood Are Determined by Priming Conditions. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1139734
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139734