Protective Effect of Edaravone against Carbon Monoxide Induced Apoptosis in Rat Primary Cultured Astrocytes

المؤلفون المشاركون

Jiang, Yuan
Xu, Xiaodan
Zhang, Hong
Wang, Ke
Tu, Tao

المصدر

Biochemistry Research International

العدد

المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-8، 8ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2017-02-02

دولة النشر

مصر

عدد الصفحات

8

التخصصات الرئيسية

الكيمياء

الملخص EN

Objective.

To observe the protective effect of edaravone (Eda) on astrocytes after prolonged exposure to carbon monoxide (CO) and further to investigate the potential mechanisms of Eda against CO-induced apoptosis.

Methods.

The rat primary cultured astrocytes were cultured in vitro and exposed to 1% CO for 24 h after being cultured with different concentrations of Eda.

MTT assay was used to detect the cytotoxicity of CO.

Flow cytometry was used to detect the apoptosis rate, membrane potential of mitochondria, and ROS level.

The mRNA and protein expressions of Bcl-2, Bax, and caspase-3 were assessed by real-time PCR and Western blotting analysis, respectively.

Results.

Eda can significantly suppress cytotoxicity of CO, and it can significantly increase membrane potential of mitochondria and Bcl-2 expressions and significantly suppress the apoptosis rate, ROS level, Bax, and caspase-3 expressions.

Conclusion.

Eda protects against CO-induced apoptosis in rat primary cultured astrocytes through decreasing ROS production and subsequently inhibiting mitochondrial apoptosis pathway.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Xu, Xiaodan& Zhang, Hong& Wang, Ke& Tu, Tao& Jiang, Yuan. 2017. Protective Effect of Edaravone against Carbon Monoxide Induced Apoptosis in Rat Primary Cultured Astrocytes. Biochemistry Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1139799

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Xu, Xiaodan…[et al.]. Protective Effect of Edaravone against Carbon Monoxide Induced Apoptosis in Rat Primary Cultured Astrocytes. Biochemistry Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1139799

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Xu, Xiaodan& Zhang, Hong& Wang, Ke& Tu, Tao& Jiang, Yuan. Protective Effect of Edaravone against Carbon Monoxide Induced Apoptosis in Rat Primary Cultured Astrocytes. Biochemistry Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1139799

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1139799