Protective Effect of Edaravone against Carbon Monoxide Induced Apoptosis in Rat Primary Cultured Astrocytes
Joint Authors
Jiang, Yuan
Xu, Xiaodan
Zhang, Hong
Wang, Ke
Tu, Tao
Source
Biochemistry Research International
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objective.
To observe the protective effect of edaravone (Eda) on astrocytes after prolonged exposure to carbon monoxide (CO) and further to investigate the potential mechanisms of Eda against CO-induced apoptosis.
Methods.
The rat primary cultured astrocytes were cultured in vitro and exposed to 1% CO for 24 h after being cultured with different concentrations of Eda.
MTT assay was used to detect the cytotoxicity of CO.
Flow cytometry was used to detect the apoptosis rate, membrane potential of mitochondria, and ROS level.
The mRNA and protein expressions of Bcl-2, Bax, and caspase-3 were assessed by real-time PCR and Western blotting analysis, respectively.
Results.
Eda can significantly suppress cytotoxicity of CO, and it can significantly increase membrane potential of mitochondria and Bcl-2 expressions and significantly suppress the apoptosis rate, ROS level, Bax, and caspase-3 expressions.
Conclusion.
Eda protects against CO-induced apoptosis in rat primary cultured astrocytes through decreasing ROS production and subsequently inhibiting mitochondrial apoptosis pathway.
American Psychological Association (APA)
Xu, Xiaodan& Zhang, Hong& Wang, Ke& Tu, Tao& Jiang, Yuan. 2017. Protective Effect of Edaravone against Carbon Monoxide Induced Apoptosis in Rat Primary Cultured Astrocytes. Biochemistry Research International،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1139799
Modern Language Association (MLA)
Xu, Xiaodan…[et al.]. Protective Effect of Edaravone against Carbon Monoxide Induced Apoptosis in Rat Primary Cultured Astrocytes. Biochemistry Research International No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1139799
American Medical Association (AMA)
Xu, Xiaodan& Zhang, Hong& Wang, Ke& Tu, Tao& Jiang, Yuan. Protective Effect of Edaravone against Carbon Monoxide Induced Apoptosis in Rat Primary Cultured Astrocytes. Biochemistry Research International. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1139799
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1139799