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Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR
المؤلفون المشاركون
Löscher, Thomas
Alberer, Martin
Schlenker, Nicklas
Bauer, Malkin
Helfrich, Kerstin
Mengele, Carolin
Nothdurft, Hans Dieter
Bretzel, Gisela
Beissner, Marcus
المصدر
Canadian Journal of Infectious Diseases and Medical Microbiology
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-03-16
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Purpose.
Up to 30% of international travelers are affected by travelers’ diarrhea (TD).
Reliable data on the etiology of TD is lacking.
Sufficient laboratory capacity at travel destinations is often unavailable and transporting conventional stool samples to the home country is inconvenient.
We evaluated the use of Hemoccult cards for stool sampling combined with a multiplex PCR for the detection of model viral, bacterial, and protozoal TD pathogens.
Methods.
Following the creation of serial dilutions for each model pathogen, last positive dilution steps (LPDs) and thereof calculated last positive sample concentrations (LPCs) were compared between conventional stool samples and card samples.
Furthermore, card samples were tested after a prolonged time interval simulating storage during a travel duration of up to 6 weeks.
Results.
The LPDs/LPCs were comparable to testing of conventional stool samples.
After storage on Hemoccult cards, the recovery rate was 97.6% for C.
jejuni, 100% for E.
histolytica, 97.6% for norovirus GI, and 100% for GII.
Detection of expected pathogens was possible at weekly intervals up to 42 days.
Conclusion.
Stool samples on Hemoccult cards stored at room temperature can be used in combination with a multiplex PCR as a reliable tool for testing of TD pathogens.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Alberer, Martin& Schlenker, Nicklas& Bauer, Malkin& Helfrich, Kerstin& Mengele, Carolin& Löscher, Thomas…[et al.]. 2017. Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1141555
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Alberer, Martin…[et al.]. Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1141555
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Alberer, Martin& Schlenker, Nicklas& Bauer, Malkin& Helfrich, Kerstin& Mengele, Carolin& Löscher, Thomas…[et al.]. Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR. Canadian Journal of Infectious Diseases and Medical Microbiology. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1141555
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1141555
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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