Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR
Joint Authors
Löscher, Thomas
Alberer, Martin
Schlenker, Nicklas
Bauer, Malkin
Helfrich, Kerstin
Mengele, Carolin
Nothdurft, Hans Dieter
Bretzel, Gisela
Beissner, Marcus
Source
Canadian Journal of Infectious Diseases and Medical Microbiology
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-16
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Purpose.
Up to 30% of international travelers are affected by travelers’ diarrhea (TD).
Reliable data on the etiology of TD is lacking.
Sufficient laboratory capacity at travel destinations is often unavailable and transporting conventional stool samples to the home country is inconvenient.
We evaluated the use of Hemoccult cards for stool sampling combined with a multiplex PCR for the detection of model viral, bacterial, and protozoal TD pathogens.
Methods.
Following the creation of serial dilutions for each model pathogen, last positive dilution steps (LPDs) and thereof calculated last positive sample concentrations (LPCs) were compared between conventional stool samples and card samples.
Furthermore, card samples were tested after a prolonged time interval simulating storage during a travel duration of up to 6 weeks.
Results.
The LPDs/LPCs were comparable to testing of conventional stool samples.
After storage on Hemoccult cards, the recovery rate was 97.6% for C.
jejuni, 100% for E.
histolytica, 97.6% for norovirus GI, and 100% for GII.
Detection of expected pathogens was possible at weekly intervals up to 42 days.
Conclusion.
Stool samples on Hemoccult cards stored at room temperature can be used in combination with a multiplex PCR as a reliable tool for testing of TD pathogens.
American Psychological Association (APA)
Alberer, Martin& Schlenker, Nicklas& Bauer, Malkin& Helfrich, Kerstin& Mengele, Carolin& Löscher, Thomas…[et al.]. 2017. Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR. Canadian Journal of Infectious Diseases and Medical Microbiology،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1141555
Modern Language Association (MLA)
Alberer, Martin…[et al.]. Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR. Canadian Journal of Infectious Diseases and Medical Microbiology No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1141555
American Medical Association (AMA)
Alberer, Martin& Schlenker, Nicklas& Bauer, Malkin& Helfrich, Kerstin& Mengele, Carolin& Löscher, Thomas…[et al.]. Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR. Canadian Journal of Infectious Diseases and Medical Microbiology. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1141555
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1141555