Contrast-Enhanced Microtomographic Characterisation of Vessels in Native Bone and Engineered Vascularised Grafts Using Ink-Gelatin Perfusion and Phosphotungstic Acid
المؤلفون المشاركون
Scherberich, A.
Sutter, Sarah
Todorov, Atanas
Ismail, Tarek
Haumer, Alexander
Fulco, Ilario
Schulz, Georg
Kaempfen, Alexandre
Martin, Ivan
Schaefer, D. J.
المصدر
Contrast Media & Molecular Imaging
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-04-23
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Objectives.
Bone ischemia and necrosis are challenging to treat, requiring investigation of native and engineered bone revascularisation processes through advanced imaging techniques.
This study demonstrates an experimental two-step method for precise bone and vessel analysis in native bones or vascularised bone grafts using X-ray microtomography (μCT), without interfering with further histological processing.
Methods.
Distally ligated epigastric arteries or veins of 6 nude rats were inserted in central channels of porous hydroxyapatite cylinders and these pedicled grafts were implanted subcutaneously.
One week later, the rats were perfused with ink-gelatin and euthanised and the femurs, tibias, and grafts were explanted.
Samples were scanned using μCT, decalcified, incubated with phosphotungstic acid (PTA) for contrast enhancement, rescanned, and processed histologically.
Results.
Contrast-enhanced μCT displayed the course and branching of native bone vessels.
Histologically, both central (−17%) and epiphyseal vessels (−58%) appeared smaller than in μCT scans.
Hydroxyapatite cylinders were thoroughly vascularised but did not display bone formation.
Grafts with a central artery had more (+58%) and smaller (−52%) vessel branches compared to grafts with a vein.
Conclusions.
We present a relatively inexpensive and easy-to-perform two-step method to analyse bone and vessels by μCT, suitable to assess a variety of bone-regenerative strategies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sutter, Sarah& Todorov, Atanas& Ismail, Tarek& Haumer, Alexander& Fulco, Ilario& Schulz, Georg…[et al.]. 2017. Contrast-Enhanced Microtomographic Characterisation of Vessels in Native Bone and Engineered Vascularised Grafts Using Ink-Gelatin Perfusion and Phosphotungstic Acid. Contrast Media & Molecular Imaging،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1141737
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sutter, Sarah…[et al.]. Contrast-Enhanced Microtomographic Characterisation of Vessels in Native Bone and Engineered Vascularised Grafts Using Ink-Gelatin Perfusion and Phosphotungstic Acid. Contrast Media & Molecular Imaging No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1141737
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sutter, Sarah& Todorov, Atanas& Ismail, Tarek& Haumer, Alexander& Fulco, Ilario& Schulz, Georg…[et al.]. Contrast-Enhanced Microtomographic Characterisation of Vessels in Native Bone and Engineered Vascularised Grafts Using Ink-Gelatin Perfusion and Phosphotungstic Acid. Contrast Media & Molecular Imaging. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1141737
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1141737
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر