Contrast-Enhanced Microtomographic Characterisation of Vessels in Native Bone and Engineered Vascularised Grafts Using Ink-Gelatin Perfusion and Phosphotungstic Acid
Joint Authors
Scherberich, A.
Sutter, Sarah
Todorov, Atanas
Ismail, Tarek
Haumer, Alexander
Fulco, Ilario
Schulz, Georg
Kaempfen, Alexandre
Martin, Ivan
Schaefer, D. J.
Source
Contrast Media & Molecular Imaging
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objectives.
Bone ischemia and necrosis are challenging to treat, requiring investigation of native and engineered bone revascularisation processes through advanced imaging techniques.
This study demonstrates an experimental two-step method for precise bone and vessel analysis in native bones or vascularised bone grafts using X-ray microtomography (μCT), without interfering with further histological processing.
Methods.
Distally ligated epigastric arteries or veins of 6 nude rats were inserted in central channels of porous hydroxyapatite cylinders and these pedicled grafts were implanted subcutaneously.
One week later, the rats were perfused with ink-gelatin and euthanised and the femurs, tibias, and grafts were explanted.
Samples were scanned using μCT, decalcified, incubated with phosphotungstic acid (PTA) for contrast enhancement, rescanned, and processed histologically.
Results.
Contrast-enhanced μCT displayed the course and branching of native bone vessels.
Histologically, both central (−17%) and epiphyseal vessels (−58%) appeared smaller than in μCT scans.
Hydroxyapatite cylinders were thoroughly vascularised but did not display bone formation.
Grafts with a central artery had more (+58%) and smaller (−52%) vessel branches compared to grafts with a vein.
Conclusions.
We present a relatively inexpensive and easy-to-perform two-step method to analyse bone and vessels by μCT, suitable to assess a variety of bone-regenerative strategies.
American Psychological Association (APA)
Sutter, Sarah& Todorov, Atanas& Ismail, Tarek& Haumer, Alexander& Fulco, Ilario& Schulz, Georg…[et al.]. 2017. Contrast-Enhanced Microtomographic Characterisation of Vessels in Native Bone and Engineered Vascularised Grafts Using Ink-Gelatin Perfusion and Phosphotungstic Acid. Contrast Media & Molecular Imaging،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1141737
Modern Language Association (MLA)
Sutter, Sarah…[et al.]. Contrast-Enhanced Microtomographic Characterisation of Vessels in Native Bone and Engineered Vascularised Grafts Using Ink-Gelatin Perfusion and Phosphotungstic Acid. Contrast Media & Molecular Imaging No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1141737
American Medical Association (AMA)
Sutter, Sarah& Todorov, Atanas& Ismail, Tarek& Haumer, Alexander& Fulco, Ilario& Schulz, Georg…[et al.]. Contrast-Enhanced Microtomographic Characterisation of Vessels in Native Bone and Engineered Vascularised Grafts Using Ink-Gelatin Perfusion and Phosphotungstic Acid. Contrast Media & Molecular Imaging. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1141737
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1141737