Presence of Circulating miR-145, miR-155, and miR-382 in Exosomes Isolated from Serum of Breast Cancer Patients and Healthy Donors
المؤلفون المشاركون
González-Cantú, Yessica M.
Rodriguez-Padilla, Cristina
Resendez-Pérez, D.
Gonzalez-Villasana, Vianey
Rashed, Mohammed H.
Bayraktar, Recep
Menchaca-Arredondo, Jorge Luis
Vazquez-Guillen, Jose Manuel
Lopez-Berestein, Gabriel
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-02-12
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
miR-145, miR-155, and miR-382 have been proposed as noninvasive biomarkers to distinguish breast cancer patients from healthy individuals.
However, it is unknown if these three miRNAs are secreted by exosomes.
Thus, we hypothesized that miR-145, miR-155, and miR-382 in breast cancer patients are present in exosomes.
We isolated exosomes from serum of breast cancer patients and healthy donors, then we characterized them according to their shape, size, and exosome markers by scanning electron microscopy, atomic force microscopy, nanoparticle tracking analysis (NTA), and Western blot and determined the exosome concentration in all samples by NTA.
Later, exosomal small RNA extraction was done to determine the expression levels of miR-145, miR-155, and miR-382 by qRT-PCR.
We observed a round shape of exosomes with a mean size of 119.84 nm in breast cancer patients and 115.4 nm in healthy donors.
All exosomes present the proteins CD63, Alix, Tsg, CD9, and CD81 commonly used as markers.
Moreover, we found a significantly high concentration of exosomes in breast cancer patients with stages I, III, and IV compared to healthy donors.
We detected miR-145, miR-155, and miR-382 in the exosomes isolated from serum of breast cancer patients and healthy donors.
Our results show that the exosomes isolated from the serum of breast cancer patients and healthy donors contains miR-145, miR-155, and miR-382 but not in a selective manner in breast cancer patients.
Moreover, our data support the association between exosome concentration and the presence of breast cancer, opening the possibility to study how miRNAs packaged into exosomes play a role in BC progression.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gonzalez-Villasana, Vianey& Rashed, Mohammed H.& González-Cantú, Yessica M.& Bayraktar, Recep& Menchaca-Arredondo, Jorge Luis& Vazquez-Guillen, Jose Manuel…[et al.]. 2019. Presence of Circulating miR-145, miR-155, and miR-382 in Exosomes Isolated from Serum of Breast Cancer Patients and Healthy Donors. Disease Markers،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1147611
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gonzalez-Villasana, Vianey…[et al.]. Presence of Circulating miR-145, miR-155, and miR-382 in Exosomes Isolated from Serum of Breast Cancer Patients and Healthy Donors. Disease Markers No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1147611
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gonzalez-Villasana, Vianey& Rashed, Mohammed H.& González-Cantú, Yessica M.& Bayraktar, Recep& Menchaca-Arredondo, Jorge Luis& Vazquez-Guillen, Jose Manuel…[et al.]. Presence of Circulating miR-145, miR-155, and miR-382 in Exosomes Isolated from Serum of Breast Cancer Patients and Healthy Donors. Disease Markers. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1147611
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1147611
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر