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Presence of Circulating miR-145, miR-155, and miR-382 in Exosomes Isolated from Serum of Breast Cancer Patients and Healthy Donors
Joint Authors
González-Cantú, Yessica M.
Rodriguez-Padilla, Cristina
Resendez-Pérez, D.
Gonzalez-Villasana, Vianey
Rashed, Mohammed H.
Bayraktar, Recep
Menchaca-Arredondo, Jorge Luis
Vazquez-Guillen, Jose Manuel
Lopez-Berestein, Gabriel
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
miR-145, miR-155, and miR-382 have been proposed as noninvasive biomarkers to distinguish breast cancer patients from healthy individuals.
However, it is unknown if these three miRNAs are secreted by exosomes.
Thus, we hypothesized that miR-145, miR-155, and miR-382 in breast cancer patients are present in exosomes.
We isolated exosomes from serum of breast cancer patients and healthy donors, then we characterized them according to their shape, size, and exosome markers by scanning electron microscopy, atomic force microscopy, nanoparticle tracking analysis (NTA), and Western blot and determined the exosome concentration in all samples by NTA.
Later, exosomal small RNA extraction was done to determine the expression levels of miR-145, miR-155, and miR-382 by qRT-PCR.
We observed a round shape of exosomes with a mean size of 119.84 nm in breast cancer patients and 115.4 nm in healthy donors.
All exosomes present the proteins CD63, Alix, Tsg, CD9, and CD81 commonly used as markers.
Moreover, we found a significantly high concentration of exosomes in breast cancer patients with stages I, III, and IV compared to healthy donors.
We detected miR-145, miR-155, and miR-382 in the exosomes isolated from serum of breast cancer patients and healthy donors.
Our results show that the exosomes isolated from the serum of breast cancer patients and healthy donors contains miR-145, miR-155, and miR-382 but not in a selective manner in breast cancer patients.
Moreover, our data support the association between exosome concentration and the presence of breast cancer, opening the possibility to study how miRNAs packaged into exosomes play a role in BC progression.
American Psychological Association (APA)
Gonzalez-Villasana, Vianey& Rashed, Mohammed H.& González-Cantú, Yessica M.& Bayraktar, Recep& Menchaca-Arredondo, Jorge Luis& Vazquez-Guillen, Jose Manuel…[et al.]. 2019. Presence of Circulating miR-145, miR-155, and miR-382 in Exosomes Isolated from Serum of Breast Cancer Patients and Healthy Donors. Disease Markers،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1147611
Modern Language Association (MLA)
Gonzalez-Villasana, Vianey…[et al.]. Presence of Circulating miR-145, miR-155, and miR-382 in Exosomes Isolated from Serum of Breast Cancer Patients and Healthy Donors. Disease Markers No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1147611
American Medical Association (AMA)
Gonzalez-Villasana, Vianey& Rashed, Mohammed H.& González-Cantú, Yessica M.& Bayraktar, Recep& Menchaca-Arredondo, Jorge Luis& Vazquez-Guillen, Jose Manuel…[et al.]. Presence of Circulating miR-145, miR-155, and miR-382 in Exosomes Isolated from Serum of Breast Cancer Patients and Healthy Donors. Disease Markers. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1147611
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1147611