Promoter Hypomethylation Is Responsible for Upregulated Expression of HAI-1 in Hepatocellular Carcinoma
المؤلفون المشاركون
Teng, Lisong
Du, Xiaoxiao
Wu, Lingyan
Ur Rahman, Muhammad Saif
Teng, Xiaodong
Ye, Jingjia
Cao, Jiang
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-08-28
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
An upregulated expression of hepatocyte growth factor activator inhibitor type 1 (HAI-1) in hepatocellular carcinomas (HCC) associates with poor prognosis, but the underlying mechanism for expression regulation has not been elucidated.
HAI-1 was expressed in HCC cell line Hep3B cells at a high level but absent or has a low level in other HCC cell lines HepG2 and SMMC7721 and immortal normal liver cell line L02 at transcriptional and translational levels, respectively.
A dual-luciferase reporter assay showed that transcriptional activity of HAI-1 in the promoter region (-452 bp to -280 bp from the mRNA start site) was strongly enhanced in Hep3B and SMMC7721.
Bisulfite genomic sequencing results of the HAI-1 promoter region showed an inverse correlation between levels of promoter methylation and expression in HCC cells.
The expression level of HAI-1 in SMMC7721, HepG2, and L02 cells was elevated after 5-Aza-2′-deoxycytidine treatment.
Hypomethylation of the HAI-1 promoter region contributed to the elevated HAI-1 expression in HCC tissues.
In addition, the hypomethylation of the HAI-1 promoter region correlated with poor differentiation status of HCC tissues.
Our findings indicate that promoter hypomethylation is an important mechanism for aberrant HAI-1 expression regulation in HCC.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Du, Xiaoxiao& Wu, Lingyan& Ur Rahman, Muhammad Saif& Teng, Xiaodong& Teng, Lisong& Ye, Jingjia…[et al.]. 2019. Promoter Hypomethylation Is Responsible for Upregulated Expression of HAI-1 in Hepatocellular Carcinoma. Disease Markers،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1147964
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Du, Xiaoxiao…[et al.]. Promoter Hypomethylation Is Responsible for Upregulated Expression of HAI-1 in Hepatocellular Carcinoma. Disease Markers No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1147964
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Du, Xiaoxiao& Wu, Lingyan& Ur Rahman, Muhammad Saif& Teng, Xiaodong& Teng, Lisong& Ye, Jingjia…[et al.]. Promoter Hypomethylation Is Responsible for Upregulated Expression of HAI-1 in Hepatocellular Carcinoma. Disease Markers. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1147964
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1147964
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر