Promoter Hypomethylation Is Responsible for Upregulated Expression of HAI-1 in Hepatocellular Carcinoma
Joint Authors
Teng, Lisong
Du, Xiaoxiao
Wu, Lingyan
Ur Rahman, Muhammad Saif
Teng, Xiaodong
Ye, Jingjia
Cao, Jiang
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-08-28
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
An upregulated expression of hepatocyte growth factor activator inhibitor type 1 (HAI-1) in hepatocellular carcinomas (HCC) associates with poor prognosis, but the underlying mechanism for expression regulation has not been elucidated.
HAI-1 was expressed in HCC cell line Hep3B cells at a high level but absent or has a low level in other HCC cell lines HepG2 and SMMC7721 and immortal normal liver cell line L02 at transcriptional and translational levels, respectively.
A dual-luciferase reporter assay showed that transcriptional activity of HAI-1 in the promoter region (-452 bp to -280 bp from the mRNA start site) was strongly enhanced in Hep3B and SMMC7721.
Bisulfite genomic sequencing results of the HAI-1 promoter region showed an inverse correlation between levels of promoter methylation and expression in HCC cells.
The expression level of HAI-1 in SMMC7721, HepG2, and L02 cells was elevated after 5-Aza-2′-deoxycytidine treatment.
Hypomethylation of the HAI-1 promoter region contributed to the elevated HAI-1 expression in HCC tissues.
In addition, the hypomethylation of the HAI-1 promoter region correlated with poor differentiation status of HCC tissues.
Our findings indicate that promoter hypomethylation is an important mechanism for aberrant HAI-1 expression regulation in HCC.
American Psychological Association (APA)
Du, Xiaoxiao& Wu, Lingyan& Ur Rahman, Muhammad Saif& Teng, Xiaodong& Teng, Lisong& Ye, Jingjia…[et al.]. 2019. Promoter Hypomethylation Is Responsible for Upregulated Expression of HAI-1 in Hepatocellular Carcinoma. Disease Markers،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1147964
Modern Language Association (MLA)
Du, Xiaoxiao…[et al.]. Promoter Hypomethylation Is Responsible for Upregulated Expression of HAI-1 in Hepatocellular Carcinoma. Disease Markers No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1147964
American Medical Association (AMA)
Du, Xiaoxiao& Wu, Lingyan& Ur Rahman, Muhammad Saif& Teng, Xiaodong& Teng, Lisong& Ye, Jingjia…[et al.]. Promoter Hypomethylation Is Responsible for Upregulated Expression of HAI-1 in Hepatocellular Carcinoma. Disease Markers. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1147964
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1147964