Jiedu Sangen Decoction Inhibits the Invasion and Metastasis of Colorectal Cancer Cells by Regulating EMT through the Hippo Signaling Pathway
المؤلفون المشاركون
Li, Zhao-Yi
Min-he, Shen
Ruan, Shanming
Zhang, Kai
Wasan, Harpreet S.
Yuan, Li
Zhou, Mengmeng
Guo, Kaibo
Shen, Fengfei
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-06-25
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Colorectal cancer (CRC) is one of the most common malignant tumors affecting the digestive tract.
Moreover, the invasion and metastasis of CRC are the main reason therapy is usually inefficient.
Decreased intercellular adhesion and enhanced cell motility induced by epithelial-mesenchymal transition (EMT) provide the basic conditions for the invasion and metastasis of the epithelial tumor cells of CRC.
The Jiedu Sangen Decoction (JSD) is a prescription that has been used for more than 50 years in the treatment of CRC in the Zhejiang Hospital of Traditional Chinese Medicine.
The aim of this study was to investigate the mechanism of JSD-triggered inhibition of invasion and metastasis in colon cancer.
In vitro, the EMT model of the SW480 cells was induced by using epithelial growth factor (50 ng/mL).
In vivo, the murine model of liver metastasis was constructed by inoculating mice with the SW480 cells.
The effects of JSD on cell migration, invasion, and proliferation were determined using the transwell assay and CCK-8 assay.
Moreover, the proteins related to the EMT process and the Hippo signaling pathway in the cancerous tissues and cell lines were determined by western blotting and immunostaining.
JSD could significantly inhibit the proliferation, migration, and invasion of CRC cells and reverse their EMT status (all, P < 0.05).
Moreover, after intervention with JSD, the levels of E-Cadherin (E-cad) increased, whereas the expression levels of N-Cadherin (N-cad), Yes-associated protein (YAP), and the transcriptional coactivator with the PDZ-binding motif (TAZ) decreased in both the SW480 cells and the tumor tissues.
In summary, JSD reversed EMT and inhibited the invasion and metastasis of CRC cells through the Hippo signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yuan, Li& Zhou, Mengmeng& Wasan, Harpreet S.& Zhang, Kai& Li, Zhao-Yi& Guo, Kaibo…[et al.]. 2019. Jiedu Sangen Decoction Inhibits the Invasion and Metastasis of Colorectal Cancer Cells by Regulating EMT through the Hippo Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1148551
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yuan, Li…[et al.]. Jiedu Sangen Decoction Inhibits the Invasion and Metastasis of Colorectal Cancer Cells by Regulating EMT through the Hippo Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1148551
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yuan, Li& Zhou, Mengmeng& Wasan, Harpreet S.& Zhang, Kai& Li, Zhao-Yi& Guo, Kaibo…[et al.]. Jiedu Sangen Decoction Inhibits the Invasion and Metastasis of Colorectal Cancer Cells by Regulating EMT through the Hippo Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1148551
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1148551
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر