Jiedu Sangen Decoction Inhibits the Invasion and Metastasis of Colorectal Cancer Cells by Regulating EMT through the Hippo Signaling Pathway
Joint Authors
Li, Zhao-Yi
Min-he, Shen
Ruan, Shanming
Zhang, Kai
Wasan, Harpreet S.
Yuan, Li
Zhou, Mengmeng
Guo, Kaibo
Shen, Fengfei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-25
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Colorectal cancer (CRC) is one of the most common malignant tumors affecting the digestive tract.
Moreover, the invasion and metastasis of CRC are the main reason therapy is usually inefficient.
Decreased intercellular adhesion and enhanced cell motility induced by epithelial-mesenchymal transition (EMT) provide the basic conditions for the invasion and metastasis of the epithelial tumor cells of CRC.
The Jiedu Sangen Decoction (JSD) is a prescription that has been used for more than 50 years in the treatment of CRC in the Zhejiang Hospital of Traditional Chinese Medicine.
The aim of this study was to investigate the mechanism of JSD-triggered inhibition of invasion and metastasis in colon cancer.
In vitro, the EMT model of the SW480 cells was induced by using epithelial growth factor (50 ng/mL).
In vivo, the murine model of liver metastasis was constructed by inoculating mice with the SW480 cells.
The effects of JSD on cell migration, invasion, and proliferation were determined using the transwell assay and CCK-8 assay.
Moreover, the proteins related to the EMT process and the Hippo signaling pathway in the cancerous tissues and cell lines were determined by western blotting and immunostaining.
JSD could significantly inhibit the proliferation, migration, and invasion of CRC cells and reverse their EMT status (all, P < 0.05).
Moreover, after intervention with JSD, the levels of E-Cadherin (E-cad) increased, whereas the expression levels of N-Cadherin (N-cad), Yes-associated protein (YAP), and the transcriptional coactivator with the PDZ-binding motif (TAZ) decreased in both the SW480 cells and the tumor tissues.
In summary, JSD reversed EMT and inhibited the invasion and metastasis of CRC cells through the Hippo signaling pathway.
American Psychological Association (APA)
Yuan, Li& Zhou, Mengmeng& Wasan, Harpreet S.& Zhang, Kai& Li, Zhao-Yi& Guo, Kaibo…[et al.]. 2019. Jiedu Sangen Decoction Inhibits the Invasion and Metastasis of Colorectal Cancer Cells by Regulating EMT through the Hippo Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1148551
Modern Language Association (MLA)
Yuan, Li…[et al.]. Jiedu Sangen Decoction Inhibits the Invasion and Metastasis of Colorectal Cancer Cells by Regulating EMT through the Hippo Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1148551
American Medical Association (AMA)
Yuan, Li& Zhou, Mengmeng& Wasan, Harpreet S.& Zhang, Kai& Li, Zhao-Yi& Guo, Kaibo…[et al.]. Jiedu Sangen Decoction Inhibits the Invasion and Metastasis of Colorectal Cancer Cells by Regulating EMT through the Hippo Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1148551
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1148551