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Metformin Promotes Neuronal Differentiation via Crosstalk between Cdk5 and Sox6 in Neuroblastoma Cells
المؤلفون المشاركون
Binlateh, Thunwa
Tanasawet, Supita
Rattanaporn, Onnicha
Sukketsiri, Wanida
Hutamekalin, Pilaiwanwadee
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-02-19
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Metformin has recently emerged as a key player in promotion of neuroblastoma differentiation and neurite outgrowth.
However, molecular mechanisms of how metformin promotes cellular differentiation have not yet been fully elucidated.
In this study, we investigated how metformin promotes cell differentiation, via an inhibition of cell proliferation, by culturing SH-SY5Y neuroblastoma cells with or without metformin.
Pretreatment with reactive oxygen species (ROS) scavenger, NAC, revealed that ROS plays a crucial role in induction of cell differentiation.
Cell differentiation was observed under various morphological criteria: extension of neuritic processes and neuronal differentiation markers.
Treatment with metformin significantly increased neurite length, number of cells with neurite, and expression of neuronal differentiation markers, β-tubulin III and tyrosine hydroxylase (TH) compared with untreated control.
Further investigation found that metformin significantly decreased Cdk5 but increased Sox6 during cell differentiation.
Analysis of the mechanism underlying these changes using Cdk5 inhibitor, roscovitine, indicated that expressions of Cdk5 and Sox6 corresponded to metformin treatment.
These results suggested that metformin produces neuronal differentiation via Cdk5 and Sox6.
In addition, phosphorylated Erk1/2 was decreased while phosphorylated Akt was increased in metformin treatment.
Taken together, these findings suggest that metformin promotes neuronal differentiation via ROS activation through Cdk5/Sox6 crosstalk, relating to Erk1/2 and Akt signaling.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Binlateh, Thunwa& Tanasawet, Supita& Rattanaporn, Onnicha& Sukketsiri, Wanida& Hutamekalin, Pilaiwanwadee. 2019. Metformin Promotes Neuronal Differentiation via Crosstalk between Cdk5 and Sox6 in Neuroblastoma Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1148687
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Binlateh, Thunwa…[et al.]. Metformin Promotes Neuronal Differentiation via Crosstalk between Cdk5 and Sox6 in Neuroblastoma Cells. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1148687
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Binlateh, Thunwa& Tanasawet, Supita& Rattanaporn, Onnicha& Sukketsiri, Wanida& Hutamekalin, Pilaiwanwadee. Metformin Promotes Neuronal Differentiation via Crosstalk between Cdk5 and Sox6 in Neuroblastoma Cells. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1148687
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1148687
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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