![](/images/graphics-bg.png)
Metformin Promotes Neuronal Differentiation via Crosstalk between Cdk5 and Sox6 in Neuroblastoma Cells
Joint Authors
Binlateh, Thunwa
Tanasawet, Supita
Rattanaporn, Onnicha
Sukketsiri, Wanida
Hutamekalin, Pilaiwanwadee
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-19
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Metformin has recently emerged as a key player in promotion of neuroblastoma differentiation and neurite outgrowth.
However, molecular mechanisms of how metformin promotes cellular differentiation have not yet been fully elucidated.
In this study, we investigated how metformin promotes cell differentiation, via an inhibition of cell proliferation, by culturing SH-SY5Y neuroblastoma cells with or without metformin.
Pretreatment with reactive oxygen species (ROS) scavenger, NAC, revealed that ROS plays a crucial role in induction of cell differentiation.
Cell differentiation was observed under various morphological criteria: extension of neuritic processes and neuronal differentiation markers.
Treatment with metformin significantly increased neurite length, number of cells with neurite, and expression of neuronal differentiation markers, β-tubulin III and tyrosine hydroxylase (TH) compared with untreated control.
Further investigation found that metformin significantly decreased Cdk5 but increased Sox6 during cell differentiation.
Analysis of the mechanism underlying these changes using Cdk5 inhibitor, roscovitine, indicated that expressions of Cdk5 and Sox6 corresponded to metformin treatment.
These results suggested that metformin produces neuronal differentiation via Cdk5 and Sox6.
In addition, phosphorylated Erk1/2 was decreased while phosphorylated Akt was increased in metformin treatment.
Taken together, these findings suggest that metformin promotes neuronal differentiation via ROS activation through Cdk5/Sox6 crosstalk, relating to Erk1/2 and Akt signaling.
American Psychological Association (APA)
Binlateh, Thunwa& Tanasawet, Supita& Rattanaporn, Onnicha& Sukketsiri, Wanida& Hutamekalin, Pilaiwanwadee. 2019. Metformin Promotes Neuronal Differentiation via Crosstalk between Cdk5 and Sox6 in Neuroblastoma Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1148687
Modern Language Association (MLA)
Binlateh, Thunwa…[et al.]. Metformin Promotes Neuronal Differentiation via Crosstalk between Cdk5 and Sox6 in Neuroblastoma Cells. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1148687
American Medical Association (AMA)
Binlateh, Thunwa& Tanasawet, Supita& Rattanaporn, Onnicha& Sukketsiri, Wanida& Hutamekalin, Pilaiwanwadee. Metformin Promotes Neuronal Differentiation via Crosstalk between Cdk5 and Sox6 in Neuroblastoma Cells. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1148687
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1148687