Chemical Composition and Antiproliferative Effects of a Methanol Extract of Aspongopus chinensis Dallas

الملخص EN

Natural products from insects can be potent sources for developing a variety of pharmaceutical products.

Aspongopus chinensis Dallas has been used as a traditional Chinese medicine and there are several clinical evidences to support its anticancer activity.

However, the anticancer active ingredients present in A.

chinensis remain unidentified.

In the present study, we investigated the anticancer effects of a methanol extract of A.

chinensis (AME).

Gas chromatography mass spectrometry was used to analyse the chemical composition of AME.

The cell viability of MDA-MB-453 and HCC-1937 cells treated with different concentrations of AME was detected by MTT assay and the ratio of cells in different cell cycle phases was analysed by flow cytometry.

The expression of genes associated with cell cycle was analysed by real-time PCR assay.

The results showed that oleic acid (25.39%) and palmitic acid (21.798%) are the main anticancer compounds present in AME.

There was a concentration-dependent decrease in the proliferation of MDA-MB-453 and HCC-1937 cells.

Moreover, treatment with AME induced a S-phase arrest in the cells.

Real-time PCR assay demonstrated that AME could significantly downregulate the expression of CDC20, AURKB, PLK1, CCNB2, and TOP2A mRNAs and upregulate the expression of GADD45A mRNA.

We demonstrate that the methanol extract of A.

chinensis could be a potential natural alternative or complementary therapy for breast cancer.