Chemical Composition and Antiproliferative Effects of a Methanol Extract of Aspongopus chinensis Dallas
Joint Authors
Tan, Jun
Tian, Ying
Cai, Renlian
Luo, Rui
Guo, Jianjun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Natural products from insects can be potent sources for developing a variety of pharmaceutical products.
Aspongopus chinensis Dallas has been used as a traditional Chinese medicine and there are several clinical evidences to support its anticancer activity.
However, the anticancer active ingredients present in A.
chinensis remain unidentified.
In the present study, we investigated the anticancer effects of a methanol extract of A.
chinensis (AME).
Gas chromatography mass spectrometry was used to analyse the chemical composition of AME.
The cell viability of MDA-MB-453 and HCC-1937 cells treated with different concentrations of AME was detected by MTT assay and the ratio of cells in different cell cycle phases was analysed by flow cytometry.
The expression of genes associated with cell cycle was analysed by real-time PCR assay.
The results showed that oleic acid (25.39%) and palmitic acid (21.798%) are the main anticancer compounds present in AME.
There was a concentration-dependent decrease in the proliferation of MDA-MB-453 and HCC-1937 cells.
Moreover, treatment with AME induced a S-phase arrest in the cells.
Real-time PCR assay demonstrated that AME could significantly downregulate the expression of CDC20, AURKB, PLK1, CCNB2, and TOP2A mRNAs and upregulate the expression of GADD45A mRNA.
We demonstrate that the methanol extract of A.
chinensis could be a potential natural alternative or complementary therapy for breast cancer.
American Psychological Association (APA)
Tan, Jun& Tian, Ying& Cai, Renlian& Luo, Rui& Guo, Jianjun. 2019. Chemical Composition and Antiproliferative Effects of a Methanol Extract of Aspongopus chinensis Dallas. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1148988
Modern Language Association (MLA)
Tan, Jun…[et al.]. Chemical Composition and Antiproliferative Effects of a Methanol Extract of Aspongopus chinensis Dallas. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1148988
American Medical Association (AMA)
Tan, Jun& Tian, Ying& Cai, Renlian& Luo, Rui& Guo, Jianjun. Chemical Composition and Antiproliferative Effects of a Methanol Extract of Aspongopus chinensis Dallas. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1148988
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1148988