Chinese Medicine FTZ Recipe Protects against High-Glucose-Induced Beta Cell Injury through Alleviating Oxidative Stress
المؤلفون المشاركون
Bei, Wei-jian
Guo, Jiao
Wang, Yujiao
Chen, Jianmei
Zhang, Jingjing
Wang, Lexun
Gu, Zhanhui
Hu, Yinming
Huang, Yijian
Xu, Wei
Lei, Zili
Cai, Jinyan
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-03-03
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Objective.
To investigate the effect of FTZ on high-glucose-induced oxidative stress and underlying mechanisms.
Methods.
We used a β cell dysfunction and diabetes model that was induced in rats fed a high-fat high-sugar diet (HFHSD) for 6 weeks and injected once with 35 mg/kg streptozocin (STZ).
Then, 3 and 6 g/kg of FTZ were administered by gavage for 8 weeks.
In addition, an ex vivo model of oxidative stress was induced by stimulating INS-1 cells with 25 mmol/L glucose for 48 h.
Result.
The levels of fasting blood glucose (FBG) in diabetic model rats were obviously higher than those in the normal group; furthermore with reduced levels of β cells, catalase (CAT), superoxide dismutase (SOD), and Bcl-2 increased lipid peroxide malondialdehyde (MDA) and caspase-3 in the pancreatic tissue of the diabetic model rats.
Afterward, the cells were incubated with FTZ-containing serum and edaravone.
The 25 mmol/L glucose-induced SOD reduction increased MDA and intracellular ROS.
The protein expression level of Mn-SOD and CAT in the model group decreased significantly compared with that in the control group.
Conclusion.
FTZ treatment significantly improved the alteration in the level of SOD, CAT, Bcl-2, caspase-3, and MDA coupled with β cell dysfunction in diabetic rats.
Oxidative stress in INS-1 cells was closely associated with a higher rate of apoptosis, increased production of ROS and MDA, enhanced Bax expression, and caspase-3, -9 activities and markedly decreased protein expression of Mn-SOD and CAT.
FTZ-containing serum incubation notably reversed the high-glucose-evoked increase in cell apoptosis, production of ROS and MDA, and Bax protein levels.
Furthermore, FTZ stimulation upregulated the expression levels of several genes, including Mn-SOD, CAT, and Bcl-2/Bcl-xl.
In addition, FTZ decreased the intracellular activity of caspase-3, -9 in INS-1 cells.
FTZ protected β-cells from oxidative stress induced by high glucose in vivo and in vitro.
The beneficial effect of FTZ was closely associated with a decrease in the activity of caspase-3, -9 and intracellular production of ROS, MDA, and Bax coupled with an increase in the expression of Mn-SOD, CAT, and Bcl-2/Bcl-xl.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bei, Wei-jian& Wang, Yujiao& Chen, Jianmei& Zhang, Jingjing& Wang, Lexun& Gu, Zhanhui…[et al.]. 2019. Chinese Medicine FTZ Recipe Protects against High-Glucose-Induced Beta Cell Injury through Alleviating Oxidative Stress. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1150487
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bei, Wei-jian…[et al.]. Chinese Medicine FTZ Recipe Protects against High-Glucose-Induced Beta Cell Injury through Alleviating Oxidative Stress. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1150487
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bei, Wei-jian& Wang, Yujiao& Chen, Jianmei& Zhang, Jingjing& Wang, Lexun& Gu, Zhanhui…[et al.]. Chinese Medicine FTZ Recipe Protects against High-Glucose-Induced Beta Cell Injury through Alleviating Oxidative Stress. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1150487
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1150487
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر