Chinese Medicine FTZ Recipe Protects against High-Glucose-Induced Beta Cell Injury through Alleviating Oxidative Stress
Joint Authors
Bei, Wei-jian
Guo, Jiao
Wang, Yujiao
Chen, Jianmei
Zhang, Jingjing
Wang, Lexun
Gu, Zhanhui
Hu, Yinming
Huang, Yijian
Xu, Wei
Lei, Zili
Cai, Jinyan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-03
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Objective.
To investigate the effect of FTZ on high-glucose-induced oxidative stress and underlying mechanisms.
Methods.
We used a β cell dysfunction and diabetes model that was induced in rats fed a high-fat high-sugar diet (HFHSD) for 6 weeks and injected once with 35 mg/kg streptozocin (STZ).
Then, 3 and 6 g/kg of FTZ were administered by gavage for 8 weeks.
In addition, an ex vivo model of oxidative stress was induced by stimulating INS-1 cells with 25 mmol/L glucose for 48 h.
Result.
The levels of fasting blood glucose (FBG) in diabetic model rats were obviously higher than those in the normal group; furthermore with reduced levels of β cells, catalase (CAT), superoxide dismutase (SOD), and Bcl-2 increased lipid peroxide malondialdehyde (MDA) and caspase-3 in the pancreatic tissue of the diabetic model rats.
Afterward, the cells were incubated with FTZ-containing serum and edaravone.
The 25 mmol/L glucose-induced SOD reduction increased MDA and intracellular ROS.
The protein expression level of Mn-SOD and CAT in the model group decreased significantly compared with that in the control group.
Conclusion.
FTZ treatment significantly improved the alteration in the level of SOD, CAT, Bcl-2, caspase-3, and MDA coupled with β cell dysfunction in diabetic rats.
Oxidative stress in INS-1 cells was closely associated with a higher rate of apoptosis, increased production of ROS and MDA, enhanced Bax expression, and caspase-3, -9 activities and markedly decreased protein expression of Mn-SOD and CAT.
FTZ-containing serum incubation notably reversed the high-glucose-evoked increase in cell apoptosis, production of ROS and MDA, and Bax protein levels.
Furthermore, FTZ stimulation upregulated the expression levels of several genes, including Mn-SOD, CAT, and Bcl-2/Bcl-xl.
In addition, FTZ decreased the intracellular activity of caspase-3, -9 in INS-1 cells.
FTZ protected β-cells from oxidative stress induced by high glucose in vivo and in vitro.
The beneficial effect of FTZ was closely associated with a decrease in the activity of caspase-3, -9 and intracellular production of ROS, MDA, and Bax coupled with an increase in the expression of Mn-SOD, CAT, and Bcl-2/Bcl-xl.
American Psychological Association (APA)
Bei, Wei-jian& Wang, Yujiao& Chen, Jianmei& Zhang, Jingjing& Wang, Lexun& Gu, Zhanhui…[et al.]. 2019. Chinese Medicine FTZ Recipe Protects against High-Glucose-Induced Beta Cell Injury through Alleviating Oxidative Stress. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1150487
Modern Language Association (MLA)
Bei, Wei-jian…[et al.]. Chinese Medicine FTZ Recipe Protects against High-Glucose-Induced Beta Cell Injury through Alleviating Oxidative Stress. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1150487
American Medical Association (AMA)
Bei, Wei-jian& Wang, Yujiao& Chen, Jianmei& Zhang, Jingjing& Wang, Lexun& Gu, Zhanhui…[et al.]. Chinese Medicine FTZ Recipe Protects against High-Glucose-Induced Beta Cell Injury through Alleviating Oxidative Stress. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1150487
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1150487