Chinese Medicine FTZ Recipe Protects against High-Glucose-Induced Beta Cell Injury through Alleviating Oxidative Stress

Abstract EN

Objective.

To investigate the effect of FTZ on high-glucose-induced oxidative stress and underlying mechanisms.

Methods.

We used a β cell dysfunction and diabetes model that was induced in rats fed a high-fat high-sugar diet (HFHSD) for 6 weeks and injected once with 35 mg/kg streptozocin (STZ).

Then, 3 and 6 g/kg of FTZ were administered by gavage for 8 weeks.

In addition, an ex vivo model of oxidative stress was induced by stimulating INS-1 cells with 25 mmol/L glucose for 48 h.

Result.

The levels of fasting blood glucose (FBG) in diabetic model rats were obviously higher than those in the normal group; furthermore with reduced levels of β cells, catalase (CAT), superoxide dismutase (SOD), and Bcl-2 increased lipid peroxide malondialdehyde (MDA) and caspase-3 in the pancreatic tissue of the diabetic model rats.

Afterward, the cells were incubated with FTZ-containing serum and edaravone.

The 25 mmol/L glucose-induced SOD reduction increased MDA and intracellular ROS.

The protein expression level of Mn-SOD and CAT in the model group decreased significantly compared with that in the control group.

Conclusion.

FTZ treatment significantly improved the alteration in the level of SOD, CAT, Bcl-2, caspase-3, and MDA coupled with β cell dysfunction in diabetic rats.

Oxidative stress in INS-1 cells was closely associated with a higher rate of apoptosis, increased production of ROS and MDA, enhanced Bax expression, and caspase-3, -9 activities and markedly decreased protein expression of Mn-SOD and CAT.

FTZ-containing serum incubation notably reversed the high-glucose-evoked increase in cell apoptosis, production of ROS and MDA, and Bax protein levels.

Furthermore, FTZ stimulation upregulated the expression levels of several genes, including Mn-SOD, CAT, and Bcl-2/Bcl-xl.

In addition, FTZ decreased the intracellular activity of caspase-3, -9 in INS-1 cells.

FTZ protected β-cells from oxidative stress induced by high glucose in vivo and in vitro.

The beneficial effect of FTZ was closely associated with a decrease in the activity of caspase-3, -9 and intracellular production of ROS, MDA, and Bax coupled with an increase in the expression of Mn-SOD, CAT, and Bcl-2/Bcl-xl.