Effect of Plastrum Testudinis Extracts on the Proliferation and Osteogenic Differentiation of rBMSCs by Regulating p38 MAPK-Related Genes

المؤلفون المشاركون

Shang, Qi
Yu, Xiang
Ren, Hui
Shen, Gengyang
Zhao, Wenhua
Zhang, Zhida
Huang, Jinjing
Yu, Peiyuan
Liang, De
Tang, Jingjing
Jiang, Xiaobing

المصدر

Evidence-Based Complementary and Alternative Medicine

العدد

المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2019-03-07

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الطب البشري

الملخص EN

Extracts from plastrum testudinis (PTE) are active compounds that have been used to treat bone diseases in traditional Chinese medicine for thousands of years.

In previous studies, we demonstrated their effects on glucocorticoid-induced osteoporosis both in vivo and in vitro.

However, the mechanisms by which PTE regulates the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rBMSCs) in vitro remain poorly understood.

In this study, rBMSCs were treated with medium (CON), PTE, osteogenic induction (OI), and a combination of PTE and OI (PTE+OI) over a 21-day period.

We found that PTE significantly promoted rBMSCs osteogenic differentiation and mineralisation after 21 days of culturing.

Moreover, PTE+OI further enhanced the differentiation and mineralisation process.

PTE upregulated STE20, IGF1R, and p38 MAPK mRNA expression and downregulated TRAF6 mRNA expression.

The extracts inhibited TRAF6 protein expression and promoted STE20, IGF1R, and phosphorylated p38 MAPK protein expression.

Our results imply that PTE promotes the proliferation and osteogenic differentiation of rBMSCs by upregulating p38 MAPK, STE20, and IGF1R and downregulating TRAF6 expression, which may provide experimental evidence of the potential of PTE in the treatment of osteoporosis.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Shang, Qi& Yu, Xiang& Ren, Hui& Shen, Gengyang& Zhao, Wenhua& Zhang, Zhida…[et al.]. 2019. Effect of Plastrum Testudinis Extracts on the Proliferation and Osteogenic Differentiation of rBMSCs by Regulating p38 MAPK-Related Genes. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1150639

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Shang, Qi…[et al.]. Effect of Plastrum Testudinis Extracts on the Proliferation and Osteogenic Differentiation of rBMSCs by Regulating p38 MAPK-Related Genes. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1150639

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Shang, Qi& Yu, Xiang& Ren, Hui& Shen, Gengyang& Zhao, Wenhua& Zhang, Zhida…[et al.]. Effect of Plastrum Testudinis Extracts on the Proliferation and Osteogenic Differentiation of rBMSCs by Regulating p38 MAPK-Related Genes. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1150639

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1150639