Effect of Plastrum Testudinis Extracts on the Proliferation and Osteogenic Differentiation of rBMSCs by Regulating p38 MAPK-Related Genes
Joint Authors
Shang, Qi
Yu, Xiang
Ren, Hui
Shen, Gengyang
Zhao, Wenhua
Zhang, Zhida
Huang, Jinjing
Yu, Peiyuan
Liang, De
Tang, Jingjing
Jiang, Xiaobing
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Extracts from plastrum testudinis (PTE) are active compounds that have been used to treat bone diseases in traditional Chinese medicine for thousands of years.
In previous studies, we demonstrated their effects on glucocorticoid-induced osteoporosis both in vivo and in vitro.
However, the mechanisms by which PTE regulates the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rBMSCs) in vitro remain poorly understood.
In this study, rBMSCs were treated with medium (CON), PTE, osteogenic induction (OI), and a combination of PTE and OI (PTE+OI) over a 21-day period.
We found that PTE significantly promoted rBMSCs osteogenic differentiation and mineralisation after 21 days of culturing.
Moreover, PTE+OI further enhanced the differentiation and mineralisation process.
PTE upregulated STE20, IGF1R, and p38 MAPK mRNA expression and downregulated TRAF6 mRNA expression.
The extracts inhibited TRAF6 protein expression and promoted STE20, IGF1R, and phosphorylated p38 MAPK protein expression.
Our results imply that PTE promotes the proliferation and osteogenic differentiation of rBMSCs by upregulating p38 MAPK, STE20, and IGF1R and downregulating TRAF6 expression, which may provide experimental evidence of the potential of PTE in the treatment of osteoporosis.
American Psychological Association (APA)
Shang, Qi& Yu, Xiang& Ren, Hui& Shen, Gengyang& Zhao, Wenhua& Zhang, Zhida…[et al.]. 2019. Effect of Plastrum Testudinis Extracts on the Proliferation and Osteogenic Differentiation of rBMSCs by Regulating p38 MAPK-Related Genes. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1150639
Modern Language Association (MLA)
Shang, Qi…[et al.]. Effect of Plastrum Testudinis Extracts on the Proliferation and Osteogenic Differentiation of rBMSCs by Regulating p38 MAPK-Related Genes. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1150639
American Medical Association (AMA)
Shang, Qi& Yu, Xiang& Ren, Hui& Shen, Gengyang& Zhao, Wenhua& Zhang, Zhida…[et al.]. Effect of Plastrum Testudinis Extracts on the Proliferation and Osteogenic Differentiation of rBMSCs by Regulating p38 MAPK-Related Genes. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1150639
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1150639