Electroacupuncture Improves Synaptic Function in SAMP8 Mice Probably via Inhibition of the AMPKeEF2KeEF2 Signaling Pathway

المؤلفون المشاركون

Dong, Weiguo
Yang, Wendan
Li, Feifei
Guo, Wanqing
Qian, Changhui
Wang, Feng
Li, Changzhen
Lin, Lan
Lin, Ruhui

المصدر

Evidence-Based Complementary and Alternative Medicine

العدد

المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2019-09-18

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الطب البشري

الملخص EN

Synaptic loss and dysfunction is associated with cognitive impairment in Alzheimer’s disease (AD).

Recent evidence indicates that the AMP-activated protein kinase (AMPK)/eukaryotic elongation factor-2 kinase (eEF2K)/eukaryotic elongation factor-2 (eEF2) pathway was implicated in synaptic plasticity in AD.

Therapeutic strategies for AD treatment are currently limited.

Here, we investigate the effects of electroacupuncture (EA) on synaptic function and the AMPK/eEF2K/eEF2 signaling pathway in male senescence-accelerated mouse-prone 8 (SAMP8) mice.

Male 7-month-old SAMP8 and SAMR1 mice (senescence-accelerated mouse resistant 1) were randomly divided into 3 groups: SAMR1 control group (Rc), SAMP8 control group (Pc), and SAMP8 electroacupuncture group (Pe).

The Pe group was treated with EA for 30 days.

Transmission electron microscopy (TEM) was used to observe the structure of synapse.

The protein and mRNA expression of synaptophysin (SYN) and postsynaptic density 95 (PSD95) was examined by immunohistochemistry, western blot, and real-time RT-PCR.

The activity of AMPK and eEF2K was studied by western blot.

Our results showed that EA ameliorated synaptic loss, increased the expression of SYN and PSD95, and inhibited AMPK activation and eEF2K activity.

Collectively, these findings suggested that the mechanisms of EA improving synaptic function in AD may be associated with the inhibition of the AMPK/eEF2K/eEF2 signaling pathway.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Dong, Weiguo& Yang, Wendan& Li, Feifei& Guo, Wanqing& Qian, Changhui& Wang, Feng…[et al.]. 2019. Electroacupuncture Improves Synaptic Function in SAMP8 Mice Probably via Inhibition of the AMPKeEF2KeEF2 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1151154

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Dong, Weiguo…[et al.]. Electroacupuncture Improves Synaptic Function in SAMP8 Mice Probably via Inhibition of the AMPKeEF2KeEF2 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1151154

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Dong, Weiguo& Yang, Wendan& Li, Feifei& Guo, Wanqing& Qian, Changhui& Wang, Feng…[et al.]. Electroacupuncture Improves Synaptic Function in SAMP8 Mice Probably via Inhibition of the AMPKeEF2KeEF2 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1151154

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1151154