Electroacupuncture Improves Synaptic Function in SAMP8 Mice Probably via Inhibition of the AMPKeEF2KeEF2 Signaling Pathway
Joint Authors
Dong, Weiguo
Yang, Wendan
Li, Feifei
Guo, Wanqing
Qian, Changhui
Wang, Feng
Li, Changzhen
Lin, Lan
Lin, Ruhui
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Synaptic loss and dysfunction is associated with cognitive impairment in Alzheimer’s disease (AD).
Recent evidence indicates that the AMP-activated protein kinase (AMPK)/eukaryotic elongation factor-2 kinase (eEF2K)/eukaryotic elongation factor-2 (eEF2) pathway was implicated in synaptic plasticity in AD.
Therapeutic strategies for AD treatment are currently limited.
Here, we investigate the effects of electroacupuncture (EA) on synaptic function and the AMPK/eEF2K/eEF2 signaling pathway in male senescence-accelerated mouse-prone 8 (SAMP8) mice.
Male 7-month-old SAMP8 and SAMR1 mice (senescence-accelerated mouse resistant 1) were randomly divided into 3 groups: SAMR1 control group (Rc), SAMP8 control group (Pc), and SAMP8 electroacupuncture group (Pe).
The Pe group was treated with EA for 30 days.
Transmission electron microscopy (TEM) was used to observe the structure of synapse.
The protein and mRNA expression of synaptophysin (SYN) and postsynaptic density 95 (PSD95) was examined by immunohistochemistry, western blot, and real-time RT-PCR.
The activity of AMPK and eEF2K was studied by western blot.
Our results showed that EA ameliorated synaptic loss, increased the expression of SYN and PSD95, and inhibited AMPK activation and eEF2K activity.
Collectively, these findings suggested that the mechanisms of EA improving synaptic function in AD may be associated with the inhibition of the AMPK/eEF2K/eEF2 signaling pathway.
American Psychological Association (APA)
Dong, Weiguo& Yang, Wendan& Li, Feifei& Guo, Wanqing& Qian, Changhui& Wang, Feng…[et al.]. 2019. Electroacupuncture Improves Synaptic Function in SAMP8 Mice Probably via Inhibition of the AMPKeEF2KeEF2 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1151154
Modern Language Association (MLA)
Dong, Weiguo…[et al.]. Electroacupuncture Improves Synaptic Function in SAMP8 Mice Probably via Inhibition of the AMPKeEF2KeEF2 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1151154
American Medical Association (AMA)
Dong, Weiguo& Yang, Wendan& Li, Feifei& Guo, Wanqing& Qian, Changhui& Wang, Feng…[et al.]. Electroacupuncture Improves Synaptic Function in SAMP8 Mice Probably via Inhibition of the AMPKeEF2KeEF2 Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1151154
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1151154