Downregulation of Aquaporin 3 Mediated the Laxative Effect in the Rat Colon by a Purified Resin Glycoside Fraction from Pharbitis Semen
المؤلفون المشاركون
Zhu, Dongrong
Chen, Chen
Bai, Lijuan
Kong, Lingyi
Luo, Jianguang
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-01-13
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Background.
Pharbitis Semen, the seeds of Pharbitis nil, is widely used as a traditional purgative medicine in China, Korea, and Japan.
This study investigated the laxative effects of a purified resin glycoside fraction obtained in our previous study from Pharbitis Semen in vivo and in vitro.
Materials and Methods.
After orally administering a purified resin glycoside fraction from Pharbitis Semen (RFP) to rats, the content of fecal water, AQP3, NF-κB, COX-2 expression, and the prostaglandin E2 (PGE2) concentrations in the colon were examined.
Moreover, human intestinal epithelial cells (HT-29) were used to investigate the mechanism of RFP decreasing the AQP3 expression.
Results.
Results obtained showed that treatment with RFP increased the feces excretion and fecal water content of rats in a dose-dependent manner.
More interestingly, AQP3 expression was suppressed by RFP treatment both in the rat colons and in HT-29 cells, while the NF-κB pathway-mediated PGE2 production was activated.
Interestingly, pretreating rats with BAY-11-7082 (NF-κB inhibitor) or indomethacin (COX-2 inhibitor) and RFP neither induced diarrhea nor decreased the AQP3 expression in the colon.
Conclusions.
The purgative property of the purified resin glycoside fraction was attributed to NF-κB activation in the colon, which increased the COX-2-mediated secretion of PGE2.
PGE2 decreased AQP3 expression which inhibits water absorbed from the intestine to the blood vessel side, resulting in the laxative effect of RFP.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhu, Dongrong& Chen, Chen& Bai, Lijuan& Kong, Lingyi& Luo, Jianguang. 2019. Downregulation of Aquaporin 3 Mediated the Laxative Effect in the Rat Colon by a Purified Resin Glycoside Fraction from Pharbitis Semen. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1151689
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhu, Dongrong…[et al.]. Downregulation of Aquaporin 3 Mediated the Laxative Effect in the Rat Colon by a Purified Resin Glycoside Fraction from Pharbitis Semen. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1151689
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhu, Dongrong& Chen, Chen& Bai, Lijuan& Kong, Lingyi& Luo, Jianguang. Downregulation of Aquaporin 3 Mediated the Laxative Effect in the Rat Colon by a Purified Resin Glycoside Fraction from Pharbitis Semen. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1151689
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1151689
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر