Downregulation of Aquaporin 3 Mediated the Laxative Effect in the Rat Colon by a Purified Resin Glycoside Fraction from Pharbitis Semen
Joint Authors
Zhu, Dongrong
Chen, Chen
Bai, Lijuan
Kong, Lingyi
Luo, Jianguang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Pharbitis Semen, the seeds of Pharbitis nil, is widely used as a traditional purgative medicine in China, Korea, and Japan.
This study investigated the laxative effects of a purified resin glycoside fraction obtained in our previous study from Pharbitis Semen in vivo and in vitro.
Materials and Methods.
After orally administering a purified resin glycoside fraction from Pharbitis Semen (RFP) to rats, the content of fecal water, AQP3, NF-κB, COX-2 expression, and the prostaglandin E2 (PGE2) concentrations in the colon were examined.
Moreover, human intestinal epithelial cells (HT-29) were used to investigate the mechanism of RFP decreasing the AQP3 expression.
Results.
Results obtained showed that treatment with RFP increased the feces excretion and fecal water content of rats in a dose-dependent manner.
More interestingly, AQP3 expression was suppressed by RFP treatment both in the rat colons and in HT-29 cells, while the NF-κB pathway-mediated PGE2 production was activated.
Interestingly, pretreating rats with BAY-11-7082 (NF-κB inhibitor) or indomethacin (COX-2 inhibitor) and RFP neither induced diarrhea nor decreased the AQP3 expression in the colon.
Conclusions.
The purgative property of the purified resin glycoside fraction was attributed to NF-κB activation in the colon, which increased the COX-2-mediated secretion of PGE2.
PGE2 decreased AQP3 expression which inhibits water absorbed from the intestine to the blood vessel side, resulting in the laxative effect of RFP.
American Psychological Association (APA)
Zhu, Dongrong& Chen, Chen& Bai, Lijuan& Kong, Lingyi& Luo, Jianguang. 2019. Downregulation of Aquaporin 3 Mediated the Laxative Effect in the Rat Colon by a Purified Resin Glycoside Fraction from Pharbitis Semen. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1151689
Modern Language Association (MLA)
Zhu, Dongrong…[et al.]. Downregulation of Aquaporin 3 Mediated the Laxative Effect in the Rat Colon by a Purified Resin Glycoside Fraction from Pharbitis Semen. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1151689
American Medical Association (AMA)
Zhu, Dongrong& Chen, Chen& Bai, Lijuan& Kong, Lingyi& Luo, Jianguang. Downregulation of Aquaporin 3 Mediated the Laxative Effect in the Rat Colon by a Purified Resin Glycoside Fraction from Pharbitis Semen. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1151689
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1151689