ELISA Development for Serum Hemeoxygenase-1 and Its Application to Patients with Acute Respiratory Distress Syndrome
المؤلفون المشاركون
Hashimoto, Satoru
Hara, Yu
Nagai, Kenjiro
Shinkai, Masaharu
Kaneko, Takeshi
Taguri, Masataka
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-18
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Background.
Hemeoxygenase-1 (HO-1) is an essential enzyme in heme catabolism and has been proposed as a biomarker of lung disease prognosis.
We modified a commercial HO-1 enzyme-linked immunosorbent assay (ELISA) kit to achieve higher sensitivity and evaluated if serum HO-1 could be a biomarker to predict the prognosis of acute respiratory distress syndrome (ARDS) patients.
Methods.
Serum samples were collected from 15 healthy volunteers to validate the modified ELISA.
In the 22 patients with ARDS who were enrolled, serum HO-1 was measured upon diagnosis (D0) and at 7 days after diagnosis (D7).
Results.
The serum HO-1 concentration could be measured in all healthy volunteers.
The intra- and interassay tests and the percentage recovery test were acceptable.
Compared with normal control subjects, patients with ARDS had significantly higher D0 HO-1 concentrations (75.4 ng/mL versus 31.7 ng/mL, P<0.001).
The 28-day survival was significantly better in patients with low D0 HO-1 (<75.8 ng/mL) than in those with high D0 HO-1 (≥75.8 ng/mL) (mortality rate: 18% versus 73%, P=0.016).
Nonsurvivors had significantly higher D0 and D7 HO-1 concentrations than survivors (P<0.05).
Conclusion.
Serum HO-1 may be a useful biomarker to predict the prognosis of patients with ARDS.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hara, Yu& Shinkai, Masaharu& Taguri, Masataka& Nagai, Kenjiro& Hashimoto, Satoru& Kaneko, Takeshi. 2018. ELISA Development for Serum Hemeoxygenase-1 and Its Application to Patients with Acute Respiratory Distress Syndrome. Canadian Respiratory Journal،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1151930
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hara, Yu…[et al.]. ELISA Development for Serum Hemeoxygenase-1 and Its Application to Patients with Acute Respiratory Distress Syndrome. Canadian Respiratory Journal No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1151930
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hara, Yu& Shinkai, Masaharu& Taguri, Masataka& Nagai, Kenjiro& Hashimoto, Satoru& Kaneko, Takeshi. ELISA Development for Serum Hemeoxygenase-1 and Its Application to Patients with Acute Respiratory Distress Syndrome. Canadian Respiratory Journal. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1151930
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1151930
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر