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ELISA Development for Serum Hemeoxygenase-1 and Its Application to Patients with Acute Respiratory Distress Syndrome
Joint Authors
Hashimoto, Satoru
Hara, Yu
Nagai, Kenjiro
Shinkai, Masaharu
Kaneko, Takeshi
Taguri, Masataka
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Hemeoxygenase-1 (HO-1) is an essential enzyme in heme catabolism and has been proposed as a biomarker of lung disease prognosis.
We modified a commercial HO-1 enzyme-linked immunosorbent assay (ELISA) kit to achieve higher sensitivity and evaluated if serum HO-1 could be a biomarker to predict the prognosis of acute respiratory distress syndrome (ARDS) patients.
Methods.
Serum samples were collected from 15 healthy volunteers to validate the modified ELISA.
In the 22 patients with ARDS who were enrolled, serum HO-1 was measured upon diagnosis (D0) and at 7 days after diagnosis (D7).
Results.
The serum HO-1 concentration could be measured in all healthy volunteers.
The intra- and interassay tests and the percentage recovery test were acceptable.
Compared with normal control subjects, patients with ARDS had significantly higher D0 HO-1 concentrations (75.4 ng/mL versus 31.7 ng/mL, P<0.001).
The 28-day survival was significantly better in patients with low D0 HO-1 (<75.8 ng/mL) than in those with high D0 HO-1 (≥75.8 ng/mL) (mortality rate: 18% versus 73%, P=0.016).
Nonsurvivors had significantly higher D0 and D7 HO-1 concentrations than survivors (P<0.05).
Conclusion.
Serum HO-1 may be a useful biomarker to predict the prognosis of patients with ARDS.
American Psychological Association (APA)
Hara, Yu& Shinkai, Masaharu& Taguri, Masataka& Nagai, Kenjiro& Hashimoto, Satoru& Kaneko, Takeshi. 2018. ELISA Development for Serum Hemeoxygenase-1 and Its Application to Patients with Acute Respiratory Distress Syndrome. Canadian Respiratory Journal،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1151930
Modern Language Association (MLA)
Hara, Yu…[et al.]. ELISA Development for Serum Hemeoxygenase-1 and Its Application to Patients with Acute Respiratory Distress Syndrome. Canadian Respiratory Journal No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1151930
American Medical Association (AMA)
Hara, Yu& Shinkai, Masaharu& Taguri, Masataka& Nagai, Kenjiro& Hashimoto, Satoru& Kaneko, Takeshi. ELISA Development for Serum Hemeoxygenase-1 and Its Application to Patients with Acute Respiratory Distress Syndrome. Canadian Respiratory Journal. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1151930
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1151930