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The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser DesorptionIonization Time-of-Flight Mass Spectrometry
المؤلفون المشاركون
Xu, Bin
He, Kun
Xu, Jing
Tang, Chuanhao
Li, Xiaoyan
Qin, Haifeng
Wang, Weixia
Wang, Hong
Wang, Zhongyuan
Li, Liangliang
Li, Zhihua
Gao, Hongjun
Liu, Xiaoqing
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-03-13
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Background.
Diagnoses of malignant pleural effusion (MPE) are a crucial problem in clinics.
In our study, we compared the peptide profiles of MPE and tuberculosis pleural effusion (TPE) to investigate the value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in diagnosis of MPE.
Material and Methods.
The 46 MPE and 32 TPE were randomly assigned to training set and validation set.
Peptides were isolated by weak cation exchange magnetic beads and peaks in the m/z range of 800–10000 Da were analyzed.
Comparing the peptide profile between 30 MPE and 22 TPE samples in training set by ClinProTools software, we screened the specific biomarkers and established a MALDI-TOF-MS classification of MPE.
Finally, the other 16 MPE and 10 TPE were included to verify the model.
We additionally determined carcinoembryonic antigen (CEA) in MPE and TPE samples using electrochemiluminescent immunoassay method.
Results.
Five peptide peaks (917.37 Da, 4469.39 Da, 1466.5 Da, 4585.21 Da, and 3216.87 Da) were selected to separate MPE and TPE by MALDI-TOF-MS.
The sensitivity, specificity, and accuracy of the classification were 93.75%, 100%, and 96.15%, respectively, after blinded test.
The sensitivity of CEA was significantly lower than MALDI-TOF-MS classification (P=0.035).
Conclusions.
The results indicate MALDI-TOF-MS is a potential method for diagnosing MPE.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xu, Jing& Xu, Bin& Tang, Chuanhao& Li, Xiaoyan& Qin, Haifeng& Wang, Weixia…[et al.]. 2017. The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser DesorptionIonization Time-of-Flight Mass Spectrometry. Disease Markers،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1152277
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xu, Jing…[et al.]. The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser DesorptionIonization Time-of-Flight Mass Spectrometry. Disease Markers No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1152277
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xu, Jing& Xu, Bin& Tang, Chuanhao& Li, Xiaoyan& Qin, Haifeng& Wang, Weixia…[et al.]. The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser DesorptionIonization Time-of-Flight Mass Spectrometry. Disease Markers. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1152277
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1152277
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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