The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser DesorptionIonization Time-of-Flight Mass Spectrometry
Joint Authors
Xu, Bin
He, Kun
Xu, Jing
Tang, Chuanhao
Li, Xiaoyan
Qin, Haifeng
Wang, Weixia
Wang, Hong
Wang, Zhongyuan
Li, Liangliang
Li, Zhihua
Gao, Hongjun
Liu, Xiaoqing
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Diagnoses of malignant pleural effusion (MPE) are a crucial problem in clinics.
In our study, we compared the peptide profiles of MPE and tuberculosis pleural effusion (TPE) to investigate the value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) in diagnosis of MPE.
Material and Methods.
The 46 MPE and 32 TPE were randomly assigned to training set and validation set.
Peptides were isolated by weak cation exchange magnetic beads and peaks in the m/z range of 800–10000 Da were analyzed.
Comparing the peptide profile between 30 MPE and 22 TPE samples in training set by ClinProTools software, we screened the specific biomarkers and established a MALDI-TOF-MS classification of MPE.
Finally, the other 16 MPE and 10 TPE were included to verify the model.
We additionally determined carcinoembryonic antigen (CEA) in MPE and TPE samples using electrochemiluminescent immunoassay method.
Results.
Five peptide peaks (917.37 Da, 4469.39 Da, 1466.5 Da, 4585.21 Da, and 3216.87 Da) were selected to separate MPE and TPE by MALDI-TOF-MS.
The sensitivity, specificity, and accuracy of the classification were 93.75%, 100%, and 96.15%, respectively, after blinded test.
The sensitivity of CEA was significantly lower than MALDI-TOF-MS classification (P=0.035).
Conclusions.
The results indicate MALDI-TOF-MS is a potential method for diagnosing MPE.
American Psychological Association (APA)
Xu, Jing& Xu, Bin& Tang, Chuanhao& Li, Xiaoyan& Qin, Haifeng& Wang, Weixia…[et al.]. 2017. The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser DesorptionIonization Time-of-Flight Mass Spectrometry. Disease Markers،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1152277
Modern Language Association (MLA)
Xu, Jing…[et al.]. The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser DesorptionIonization Time-of-Flight Mass Spectrometry. Disease Markers No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1152277
American Medical Association (AMA)
Xu, Jing& Xu, Bin& Tang, Chuanhao& Li, Xiaoyan& Qin, Haifeng& Wang, Weixia…[et al.]. The Exploration of Peptide Biomarkers in Malignant Pleural Effusion of Lung Cancer Using Matrix-Assisted Laser DesorptionIonization Time-of-Flight Mass Spectrometry. Disease Markers. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1152277
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1152277