Overexpression of miR-24 Is Involved in the Formation of Hypocoagulation State after Severe Trauma by Inhibiting the Synthesis of Coagulation Factor X

الملخص EN

Background.

Dysregulation of microRNAs may contribute to the progression of trauma-induced coagulopathy (TIC).

We aimed to explore the biological function that miRNA-24-3p (miR-24) might have in coagulation factor deficiency after major trauma and TIC.

Methods.

15 healthy volunteers and 36 severe trauma patients (Injury Severity Score ≥ 16 were enrolled.

TIC was determined as the initial international normalized ratio >1.5.

The miR-24 expression and concentrations of factor X (FX) and factor XII in plasma were measured.

In vitro study was conducted on L02 cell line.

Results.

The plasma miR-24 expression was significantly elevated by 3.17-fold (P=0.043) in major trauma patients and reduced after 3 days (P<0.01).

The expression level was significantly higher in TIC than in non-TIC patients (P=0.040).

Multivariate analysis showed that the higher miR-24 expression was associated with TIC.

The plasma concentration of FX in TIC patients was significantly lower than in the non-TIC ones (P=0.030) and controls (P<0.01).

A negative correlation was observed between miR-24 and FX.

miR-24 transduction significantly reduced the FX level in the supernatant of L02 cells (P=0.030).

Conclusions.

miR-24 was overexpressed in major trauma and TIC patients.

The negative correlation of miR-24 with FX suggested the possibility that miR-24 might inhibit the synthesis of FX during TIC.