Pingmu Decoction Induces Orbital Preadipocytes Apoptosis In Vitro
المؤلفون المشاركون
Xie, Rui-Fang
Zhang, Yali
Li, Hong
Gao, Long
Zhang, Xia
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-04-18
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Pingmu Decoction is the Traditional Chinese Medicine which has treated Graves’ Ophthalmopathy (GO) in the inactive stage for more than ten years.
This study was to explore the mechanism of Pingmu Decoction of inhibiting preadipocytes in GO patients from differentiating into mature adipocytes.
Human orbital preadipocytes were isolated and cultured through tissue explant method.
Orbital preadipocytes were induced into mature adipocytes.
The medicinal serum was prepared from rats.
The cells were treated with medicinal serum which were divided into three groups, low dose group (5%), medium dose group (10%), and high dose group (20%).
The cells viabilities were observed by Oil Red O staining, MTT method, and Annexin V/propidium iodide (PI) double staining.
Effect of Pingmu Decoction on cell apoptosis rate of orbital matured adipocytes was measured by flow cytometry.
The genes Fas and Fas L from cell groups were tested by RT-PCR and Western blotting.
The expression of master adipogenic transcription factors, including peroxisome proliferation-activity receptor (PPAR) γ and CCAAT/enhancer binding protein (C/EBP) α, was tested by Western blotting.
Pingmu Decoction could reduce orbital preadipocytes viability and induce apoptosis of mature adipocyte via Fas/Fas L signaling pathway.
Pingmu Decoction reduced lipid accumulation and downregulated the expression of PPAR γ and C/EBP α.
Pingmu Decoction may play a therapeutic effect by reducing the accumulation of orbital adipocytes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Yali& Li, Hong& Gao, Long& Zhang, Xia& Xie, Rui-Fang. 2017. Pingmu Decoction Induces Orbital Preadipocytes Apoptosis In Vitro. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1153221
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Yali…[et al.]. Pingmu Decoction Induces Orbital Preadipocytes Apoptosis In Vitro. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1153221
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Yali& Li, Hong& Gao, Long& Zhang, Xia& Xie, Rui-Fang. Pingmu Decoction Induces Orbital Preadipocytes Apoptosis In Vitro. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1153221
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1153221
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر