Pingmu Decoction Induces Orbital Preadipocytes Apoptosis In Vitro
Joint Authors
Xie, Rui-Fang
Zhang, Yali
Li, Hong
Gao, Long
Zhang, Xia
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Pingmu Decoction is the Traditional Chinese Medicine which has treated Graves’ Ophthalmopathy (GO) in the inactive stage for more than ten years.
This study was to explore the mechanism of Pingmu Decoction of inhibiting preadipocytes in GO patients from differentiating into mature adipocytes.
Human orbital preadipocytes were isolated and cultured through tissue explant method.
Orbital preadipocytes were induced into mature adipocytes.
The medicinal serum was prepared from rats.
The cells were treated with medicinal serum which were divided into three groups, low dose group (5%), medium dose group (10%), and high dose group (20%).
The cells viabilities were observed by Oil Red O staining, MTT method, and Annexin V/propidium iodide (PI) double staining.
Effect of Pingmu Decoction on cell apoptosis rate of orbital matured adipocytes was measured by flow cytometry.
The genes Fas and Fas L from cell groups were tested by RT-PCR and Western blotting.
The expression of master adipogenic transcription factors, including peroxisome proliferation-activity receptor (PPAR) γ and CCAAT/enhancer binding protein (C/EBP) α, was tested by Western blotting.
Pingmu Decoction could reduce orbital preadipocytes viability and induce apoptosis of mature adipocyte via Fas/Fas L signaling pathway.
Pingmu Decoction reduced lipid accumulation and downregulated the expression of PPAR γ and C/EBP α.
Pingmu Decoction may play a therapeutic effect by reducing the accumulation of orbital adipocytes.
American Psychological Association (APA)
Zhang, Yali& Li, Hong& Gao, Long& Zhang, Xia& Xie, Rui-Fang. 2017. Pingmu Decoction Induces Orbital Preadipocytes Apoptosis In Vitro. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1153221
Modern Language Association (MLA)
Zhang, Yali…[et al.]. Pingmu Decoction Induces Orbital Preadipocytes Apoptosis In Vitro. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1153221
American Medical Association (AMA)
Zhang, Yali& Li, Hong& Gao, Long& Zhang, Xia& Xie, Rui-Fang. Pingmu Decoction Induces Orbital Preadipocytes Apoptosis In Vitro. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1153221
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153221