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scFv against HSP60 of Strongyloides sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly
المؤلفون المشاركون
de Oliveira, Carlo Jose Freire
Rodrigues, Wellington Francisco
Miguel, Camila Botelho
Goulart, Luiz Ricardo
Levenhagen, Marcelo Arantes
Costa-Cruz, Julia Maria
Alves, Patrícia Terra
Ueira-Vieira, Carlos
Brito, Patrícia Kellen Martins Oliveira
Gomes, Angelica Oliveira
Lazo-Chica, Javier Emilio
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-01-11
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
The present study is aimed at evaluating serological method using scFv anti-Strongyloides sp.
and reporting the frequencies of the results with conventional parasitological technique (faeces) in elderly individuals.
Among 112 elderly individuals (≥60 years of age), 14.28% were positive for at least one enteroparasite, with one individual positive for S.
stercoralis.
Sera were evaluated for the presence of anti-Strongyloides sp.
antibodies using total or detergent fraction extracts of Strongyloides venezuelensis, which presented positivity rates of 19.64% and 10.71%, respectively.
An anti-HSP60 single-chain variable fragment from Strongyloides sp.
was used to detect parasite antigens, with 5.36% (6 individuals) of ELISA-positive individuals returning a positive result.
While the serological test indicates previous or recent infection and may be limited by antigen purification, the anti-HSP60 method reflects the presence of Strongyloides sp.
immune complexes and exhibits greater sensitivity and specificity.
Our results demonstrate the variable occurrence of enteroparasites in elderly individuals residing in long-term nursing homes and validate a novel epidemiological tool to describe infection cases by Strongyloides sp.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Miguel, Camila Botelho& Levenhagen, Marcelo Arantes& Costa-Cruz, Julia Maria& Goulart, Luiz Ricardo& Alves, Patrícia Terra& Ueira-Vieira, Carlos…[et al.]. 2020. scFv against HSP60 of Strongyloides sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly. Disease Markers،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1153835
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Miguel, Camila Botelho…[et al.]. scFv against HSP60 of Strongyloides sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly. Disease Markers No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1153835
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Miguel, Camila Botelho& Levenhagen, Marcelo Arantes& Costa-Cruz, Julia Maria& Goulart, Luiz Ricardo& Alves, Patrícia Terra& Ueira-Vieira, Carlos…[et al.]. scFv against HSP60 of Strongyloides sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly. Disease Markers. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1153835
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1153835
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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