scFv against HSP60 of Strongyloides sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly
Joint Authors
de Oliveira, Carlo Jose Freire
Rodrigues, Wellington Francisco
Miguel, Camila Botelho
Goulart, Luiz Ricardo
Levenhagen, Marcelo Arantes
Costa-Cruz, Julia Maria
Alves, Patrícia Terra
Ueira-Vieira, Carlos
Brito, Patrícia Kellen Martins Oliveira
Gomes, Angelica Oliveira
Lazo-Chica, Javier Emilio
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-11
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The present study is aimed at evaluating serological method using scFv anti-Strongyloides sp.
and reporting the frequencies of the results with conventional parasitological technique (faeces) in elderly individuals.
Among 112 elderly individuals (≥60 years of age), 14.28% were positive for at least one enteroparasite, with one individual positive for S.
stercoralis.
Sera were evaluated for the presence of anti-Strongyloides sp.
antibodies using total or detergent fraction extracts of Strongyloides venezuelensis, which presented positivity rates of 19.64% and 10.71%, respectively.
An anti-HSP60 single-chain variable fragment from Strongyloides sp.
was used to detect parasite antigens, with 5.36% (6 individuals) of ELISA-positive individuals returning a positive result.
While the serological test indicates previous or recent infection and may be limited by antigen purification, the anti-HSP60 method reflects the presence of Strongyloides sp.
immune complexes and exhibits greater sensitivity and specificity.
Our results demonstrate the variable occurrence of enteroparasites in elderly individuals residing in long-term nursing homes and validate a novel epidemiological tool to describe infection cases by Strongyloides sp.
American Psychological Association (APA)
Miguel, Camila Botelho& Levenhagen, Marcelo Arantes& Costa-Cruz, Julia Maria& Goulart, Luiz Ricardo& Alves, Patrícia Terra& Ueira-Vieira, Carlos…[et al.]. 2020. scFv against HSP60 of Strongyloides sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly. Disease Markers،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1153835
Modern Language Association (MLA)
Miguel, Camila Botelho…[et al.]. scFv against HSP60 of Strongyloides sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly. Disease Markers No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1153835
American Medical Association (AMA)
Miguel, Camila Botelho& Levenhagen, Marcelo Arantes& Costa-Cruz, Julia Maria& Goulart, Luiz Ricardo& Alves, Patrícia Terra& Ueira-Vieira, Carlos…[et al.]. scFv against HSP60 of Strongyloides sp. and Its Application in the Evaluation of Parasite Frequency in the Elderly. Disease Markers. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1153835
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153835