Curcumin Induces Autophagy, Apoptosis, and Cell Cycle Arrest in Human Pancreatic Cancer Cells
المؤلفون المشاركون
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-09-10
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Objective.
Curcumin is an active extract from turmeric.
The aim of this study was to identify the underlying mechanism of curcumin on PCa cells and the role of autophagy in this process.
Methods.
The inhibitory effect of curcumin on the growth of PANC1 and BxPC3 cell lines was detected by CCK-8 assay.
Cell cycle distribution and apoptosis were tested by flow cytometry.
Autophagosomes were tested by cell immunofluorescence assay.
The protein expression was detected by Western blot.
The correlation between LC3II/Bax and cell viability was analyzed.
Results.
Curcumin inhibited the cell proliferation in a dose- and time-dependent manner.
Curcumin could induce cell cycle arrest at G2/M phase and apoptosis of PCa cells.
The autophagosomes were detected in the dosing groups.
Protein expression of Bax and LC3II was upregulated, while Bcl2 was downregulated in the high dosing groups of curcumin.
There was a significant negative correlation between LC3II/Bax and cell viability.
Conclusions.
Autophagy could be triggered by curcumin in the treatment of PCa.
Apoptosis and cell cycle arrest also participated in this process.
These findings imply that curcumin is a multitargeted agent for PCa cells.
In addition, autophagic cell death may predominate in the high concentration groups of curcumin.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhu, Yaping& Bu, Shurui. 2017. Curcumin Induces Autophagy, Apoptosis, and Cell Cycle Arrest in Human Pancreatic Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1154110
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhu, Yaping& Bu, Shurui. Curcumin Induces Autophagy, Apoptosis, and Cell Cycle Arrest in Human Pancreatic Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1154110
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhu, Yaping& Bu, Shurui. Curcumin Induces Autophagy, Apoptosis, and Cell Cycle Arrest in Human Pancreatic Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1154110
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1154110
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر