Curcumin Induces Autophagy, Apoptosis, and Cell Cycle Arrest in Human Pancreatic Cancer Cells
Joint Authors
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-10
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Objective.
Curcumin is an active extract from turmeric.
The aim of this study was to identify the underlying mechanism of curcumin on PCa cells and the role of autophagy in this process.
Methods.
The inhibitory effect of curcumin on the growth of PANC1 and BxPC3 cell lines was detected by CCK-8 assay.
Cell cycle distribution and apoptosis were tested by flow cytometry.
Autophagosomes were tested by cell immunofluorescence assay.
The protein expression was detected by Western blot.
The correlation between LC3II/Bax and cell viability was analyzed.
Results.
Curcumin inhibited the cell proliferation in a dose- and time-dependent manner.
Curcumin could induce cell cycle arrest at G2/M phase and apoptosis of PCa cells.
The autophagosomes were detected in the dosing groups.
Protein expression of Bax and LC3II was upregulated, while Bcl2 was downregulated in the high dosing groups of curcumin.
There was a significant negative correlation between LC3II/Bax and cell viability.
Conclusions.
Autophagy could be triggered by curcumin in the treatment of PCa.
Apoptosis and cell cycle arrest also participated in this process.
These findings imply that curcumin is a multitargeted agent for PCa cells.
In addition, autophagic cell death may predominate in the high concentration groups of curcumin.
American Psychological Association (APA)
Zhu, Yaping& Bu, Shurui. 2017. Curcumin Induces Autophagy, Apoptosis, and Cell Cycle Arrest in Human Pancreatic Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1154110
Modern Language Association (MLA)
Zhu, Yaping& Bu, Shurui. Curcumin Induces Autophagy, Apoptosis, and Cell Cycle Arrest in Human Pancreatic Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1154110
American Medical Association (AMA)
Zhu, Yaping& Bu, Shurui. Curcumin Induces Autophagy, Apoptosis, and Cell Cycle Arrest in Human Pancreatic Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1154110
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154110