Transient Expression of Lumbrokinase (PI239) in Tobacco (Nicotiana tabacum) Using a Geminivirus-Based Single Replicon System Dissolves Fibrin and Blood Clots
المؤلفون المشاركون
Tull, Lauren
Dickey, Alexia
Breedlove, Drew
Mason, Hugh S.
Wang, Nan
Wang, Kevin Yueju
Liu, Dehu
Cooper, Edwin L.
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-08-28
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Lumbrokinases, a group of fibrinolytic enzymes extracted from earthworm, have been widely used to prevent and treat various cardiovascular diseases.
They specifically target fibrin to effectively degrade thrombi without major side effects.
Plant expression systems are becoming potential alternative expression platforms for producing pharmaceutical proteins.
In this work, a lumbrokinase (PI239) was produced from a plant system.
Both wild-type (WT) and plant codon-optimized (OP) PI239 gene sequences were synthesized and cloned into a geminivirus-based single-vector DNA replicon system.
Both vectors were independently expressed in tobacco (Nicotiana tabacum) leaves transiently by agroinfiltration.
Overexpressed PI239 resulted in sudden tissue necrosis 3 days after infiltration.
Remaining proteins were purified through His-tag affinity chromatography and analyzed with SDS-PAGE and Western blot methods.
Purified PI239 successfully degraded artificial fibrin with relative activity of 13,400 U/mg when compared with commercial lumbrokinase product.
In vitro tests demonstrated that plant-derived PI239 dissolved human blood clots and that the plant expression system is capable of producing functional PI239.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Dickey, Alexia& Wang, Nan& Cooper, Edwin L.& Tull, Lauren& Breedlove, Drew& Mason, Hugh S.…[et al.]. 2017. Transient Expression of Lumbrokinase (PI239) in Tobacco (Nicotiana tabacum) Using a Geminivirus-Based Single Replicon System Dissolves Fibrin and Blood Clots. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1154212
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Dickey, Alexia…[et al.]. Transient Expression of Lumbrokinase (PI239) in Tobacco (Nicotiana tabacum) Using a Geminivirus-Based Single Replicon System Dissolves Fibrin and Blood Clots. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1154212
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Dickey, Alexia& Wang, Nan& Cooper, Edwin L.& Tull, Lauren& Breedlove, Drew& Mason, Hugh S.…[et al.]. Transient Expression of Lumbrokinase (PI239) in Tobacco (Nicotiana tabacum) Using a Geminivirus-Based Single Replicon System Dissolves Fibrin and Blood Clots. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1154212
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1154212
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر