Transient Expression of Lumbrokinase (PI239) in Tobacco (Nicotiana tabacum) Using a Geminivirus-Based Single Replicon System Dissolves Fibrin and Blood Clots
Joint Authors
Tull, Lauren
Dickey, Alexia
Breedlove, Drew
Mason, Hugh S.
Wang, Nan
Wang, Kevin Yueju
Liu, Dehu
Cooper, Edwin L.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Lumbrokinases, a group of fibrinolytic enzymes extracted from earthworm, have been widely used to prevent and treat various cardiovascular diseases.
They specifically target fibrin to effectively degrade thrombi without major side effects.
Plant expression systems are becoming potential alternative expression platforms for producing pharmaceutical proteins.
In this work, a lumbrokinase (PI239) was produced from a plant system.
Both wild-type (WT) and plant codon-optimized (OP) PI239 gene sequences were synthesized and cloned into a geminivirus-based single-vector DNA replicon system.
Both vectors were independently expressed in tobacco (Nicotiana tabacum) leaves transiently by agroinfiltration.
Overexpressed PI239 resulted in sudden tissue necrosis 3 days after infiltration.
Remaining proteins were purified through His-tag affinity chromatography and analyzed with SDS-PAGE and Western blot methods.
Purified PI239 successfully degraded artificial fibrin with relative activity of 13,400 U/mg when compared with commercial lumbrokinase product.
In vitro tests demonstrated that plant-derived PI239 dissolved human blood clots and that the plant expression system is capable of producing functional PI239.
American Psychological Association (APA)
Dickey, Alexia& Wang, Nan& Cooper, Edwin L.& Tull, Lauren& Breedlove, Drew& Mason, Hugh S.…[et al.]. 2017. Transient Expression of Lumbrokinase (PI239) in Tobacco (Nicotiana tabacum) Using a Geminivirus-Based Single Replicon System Dissolves Fibrin and Blood Clots. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1154212
Modern Language Association (MLA)
Dickey, Alexia…[et al.]. Transient Expression of Lumbrokinase (PI239) in Tobacco (Nicotiana tabacum) Using a Geminivirus-Based Single Replicon System Dissolves Fibrin and Blood Clots. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1154212
American Medical Association (AMA)
Dickey, Alexia& Wang, Nan& Cooper, Edwin L.& Tull, Lauren& Breedlove, Drew& Mason, Hugh S.…[et al.]. Transient Expression of Lumbrokinase (PI239) in Tobacco (Nicotiana tabacum) Using a Geminivirus-Based Single Replicon System Dissolves Fibrin and Blood Clots. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1154212
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154212